Phenotype Image Detail

Image

Caption

Phenotypic series of adrenomedullin signaling-null mice. Genetic deletion of genes responsible for adrenomedullin (AM) signaling results in a phenotypic series of null mice with generalized edema and lethality at mid-gestation. (A) Generalized interstitial edema, without hemorrhage, was observed throughout Calcrl^tm1Kmca/Calcrl^tm1Kmca (calcrl^-/-) embryos at E12.5. Similar edema was present by E13.5 in Adm^tm1Unc/Adm^tm1Unc (AM^-/-) embryos (D) and by E14.5 in Ramp2^tm1Kmca/Ramp2^tm1Kmca (RAMP2^-/-) embryos (G). Edema was not observed 1 day before onset, and no viable embryos were recovered 1-2 days after onset. Hematoxylin and eosin stained transverse sections of wild type (+/+) littermates (B,E and H) and Calcrl^tm1Kmca/Calcrl^tm1Kmca (C), Adm^tm1Unc/Adm^tm1Unc (F), and Ramp2^tm1Kmca/Ramp2^tm1Kmca (I) embryos at E12.5, E13.5 and E14.5, respectively. Arrowheads indicate interstitial fluid accumulation. Original magnification, A,D and G, x10; B,C,E,F,H and I, x20.

Copyright

This image is from Fritz-Six KL, J Clin Invest 2008 Jan;118(1):40-50 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:130930

Associated Alleles

Symbol	Name
Adm^tm1Unc	adrenomedullin; targeted mutation 1, University of North Carolina
Calcrl^tm1Kmca	calcitonin receptor-like; targeted mutation 1, Kathleen M Caron
Ramp2^tm1Kmca	receptor (calcitonin) activity modifying protein 2; targeted mutation 1, Kathleen Caron

Associated Genotypes

Allelic Composition	Genetic Background
Calcrl^tm1Kmca/Calcrl^tm1Kmca	129S6/SvEvTac-Calcrl^tm1Kmca
Ramp2^tm1Kmca/Ramp2^tm1Kmca	involves: 129S6/SvEvTac * C57BL/6
Adm^tm1Unc/Adm^tm1Unc	129S6/SvEvTac-Adm^tm1Unc