Phenotype Image Detail

Image

Caption

A: Scanning electron micrographs of the eyes of wild type (WT, top) and Rock1^tm1Sna/Rock1^tm1Sna (KO, middle and bottom) embryos from 14.5 to 16.5 dpc. Bidirectional arrows indicate extension of the eyelid rim. Arrowheads indicate eyelid fusion in the wild type embryos. Bar, 500um. B: Hematoxylin and eosin staining of transverse eye sections from wild type and mutant embryos from 14.5 to 18.5 dpc. Arrows indicate the eyelid epithelial sheet extending from the rim of the eyelid in the wild type embryos. Eyelid epithelial extension was impaired in the mutant embryos, with arrowheads indicating the cell mass at the expected site of sheet formation. Bar, 200um. Boxed regions 1-3 are also enlarged. Bar 20um. C: Immunofluorescence staining (green) for keratins 5, 10 and 6 as well as for Ki67 in frozen sections of the eyelids of wild type or mutant embryos at 16.0 dpc. Nuclei are stained blue. Large arrows indicate the eyelid epithelial sheet; arrowheads indicate the epithelial cell mass. The two small arrows indicate the base of the eyelid epithelial sheet. Bar, 50um.

Copyright

This image is from Shimizu Y, J Cell Biol 2005 Mar 14;168(6):941-53, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:98250

Associated Alleles

Symbol	Name
Rock1^tm1Sna	Rho-associated coiled-coil containing protein kinase 1; targeted mutation 1, Shuh Narumiya

Associated Genotypes

Allelic Composition	Genetic Background
Rock1^tm1Sna/Rock1^tm1Sna	B6N.129X1-Rock1^tm1Sna