Phenotype Image Detail

Image

Caption

A-C: Scanning electron microscopy shows that the neural tube is closed in wild type (+/+, A) and Hspg2^tm1Ref/Hspg2^tm1Ref (-/-, B and C) E10.5 embryos. Some mutant embryos show holes (C, arrows) in the fore- and midbrain and collapsed brain vesicles. D-F: High magnification of the surface ectoderm. In wild type embryos (D), the cephalic region is covered with an intact layer of ectodermal cells. Mutant embryos (E) show small clefts that are 20-30um in width and contain round cells with small extensions (arrow). In other mutant embryos (F), round cells with extensions burst through the surface ectoderm (arrow). G shows an antibody coupled to colloidal gold reacting with purified laminin. H-K: Back-scattered electron analysis shows that the small defects in the ectoderm of normal mice caused during the preparation are not labeled with the gold-conjugated antibody (secondary electron image H and backscattered electron image J), while the clefts in mutant embryos are labeled (secondary image I, backscattered electron image K). Arrow in K indicates an ectodermal defect caused during the preparation. Scale bars: D-F and H-K, 20um; G, 50nm.

Copyright

This image is from Costell M, J Cell Biol 1999 Nov 29;147(5):1109-22, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:58700

Associated Alleles

Symbol	Name
Hspg2^tm1Ref	perlecan (heparan sulfate proteoglycan 2); targeted mutation 1, Reinhard Fassler

Associated Genotypes

Allelic Composition	Genetic Background
Hspg2^tm1Ref/Hspg2^tm1Ref	involves: 129S1/Sv * 129X1/SvJ