Phenotype Image Detail

Image

Caption

A: Hematoxylin and eosin stained sagittal section through the knee joint of a 2 month old Prg4^tm1Mawa/Prg4⁺ mouse (heterozygous and wild type mice have similar histology). Femur (f), tibia (t), and synovium (arrow) are noted. B: Similar section from a Prg4^tm1Mawa/Prg4^tm1Mawa mouse (magnification in A and B, x100). Note thickening of the synovium and irregularity and thickening of the cartilage surface. Also note the irregularity in the tibial growth plate. The irregularity of the growth plate is most likely due to a secondary consequence of pathology within the joint, since lubricin is not normally expressed by growth plate chondrocytes . C and D: Hematoxylin and eosin stained sagittal sections through the knee joint of 9 month old heterozygous and homozygous mice, respectively (magnification, x100). Note the preservation of recognizable structures in the heterozygous joint in contrast to the mutant joint. Also note the hyperplastic synovium throughout the joint (arrows). E and F: Safranin-O- and fast green-stained sagittal sections through the ankle joint of 7 month old heterozyous and homozygous mice, respectively (magnification x25). Tibia, talus, calcaneus (c), tibial osteophyte (o), tibialis anterior tendon (*) and tendon sheath (ts), and synovium (arrow) are labeled. Note the abnormal appearance of the tendon, tendon sheath, synovium, and osteophyte in the homozygous mutant mouse.

Copyright

This image is from Rhee DK, J Clin Invest 2005 Mar;115(3):622-31 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:96756

Associated Alleles

Symbol	Name
Prg4^tm1Mawa	proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein); targeted mutation 1, Matthew Warman

Associated Genotypes

Allelic Composition	Genetic Background
Prg4^tm1Mawa/Prg4^tm1Mawa	involves: 129S1/Sv * 129X1/SvJ