Phenotype Image Detail

Image

Caption

Myofibrillar ultrastructure in the developing ventricular mycardium of wild-type (WT) (A,C,E) and Calr^tm1Mlk/Calr^tm1Mlk (KO) (B,D,F) mouse hearts. Magnification 10,000x. At 12.5 dpc, the ventricular myofibrils of both phenotypes are only a few sarcomeres in length (A and B). These early myofibrils exhibit disarray, which is considerably more pronounced in the mutant (B) than in the wild type (A) ventricular myocardium. At 14.5 dpc, ventricular myofibrils of wild type (C) and to a much lesser extent the mutant (D) become less disarrayed, and start to align with the long axis of the cardiomyocyte. At 18.5 dpc, most of the ventricular myofibrils of wild type phenotype (E) run in straight courses aligned parallel to each other with their Z-lines in register, thus showing little if any myofibrillar disarray. Even though mutant myofibrils become less disarrayed with embryonic development (F), their Z-lines are frequently not aligned (F). Mutant ventricular myofibrils, already wavier in their appearance than the corresponding wild type ventricular myofibrils by 13.5 dpc, do not straighten with further embryonic development as their counterparts but become visibly wavier (C vs D). This difference in the degree of waviness is most noticeable at the latest stage of embryonic development investigated, 18.5 dpc (E vs F).

Copyright

This image is from Lozyk MD, BMC Dev Biol 2006;6():54, an open-access article, licensee BioMed Central Ltd. J:119647

Associated Alleles

Symbol	Name
Calr^tm1Mlk	calreticulin; targeted mutation 1, Marek Michalak

Associated Genotypes

Allelic Composition	Genetic Background
Calr^tm1Mlk/Calr^tm1Mlk	involves: 129S4/SvJae * C57BL/6J