Gene Expression Image Detail

Reference

Figure

ID

J:78683 Hirai S, et al., Development. 2002 Oct;129(19):4483-95

2

MGI:2386226

Image

Caption

A: Western blot analysis: UV: NIH3T3 cells with UV radiation; S: serum stimulation; T: E16 telencephalic vesicles. Arrowheads indicate protein bands corresponding to each enzyme. B: Immunohistochemical staining of frozen sections using alkaline phosphatase-conjugated secondary antibody. Serial sagittal sections E16 embryos (a-e) and E18 brain (f,g) were immunostained. (e) control normal rabbit IgG. Arrows indicate the intermediate zone. The arrowhead indicates the cortical plate. Ventricular zone (VZ), intermediate zone (IZ), and cortical plate (CP) are indicated. C: The cortical region was dissected into three layers, mainly composed of the ventricular zone (V), intermediate zone (I), and cortical plate (C) from frozen sections from E16 telencephalon (T). Proteins in each layer were analyzed by western blotting (a-c, e,f) or stained with Coomassie Brilliant Blue (d). (D) in-gel kinase assay.

Copyright

This image is from Hirai et al., Development 129, 4483-4495 (2002), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A(a) JNK	MGI:3655069 (Mapk8) MGI:3655073 (Mapk9)
A(b) p38	MGI:3655074 (Mapk14)
A(c) ERK	MGI:3655077 (Mapk1) MGI:3655076 (Mapk3)
B(a)	MGI:3655092 (Map3k12)
B(b)	MGI:3655094 (Mapk8) MGI:3655095 (Mapk9)
B(c)	MGI:3655096 (Mapk14)
B(d)	MGI:3655097 (Mapk1) MGI:3655098 (Mapk3)
B(f)	MGI:3655092 (Map3k12)
B(g)	MGI:3655094 (Mapk8) MGI:3655095 (Mapk9)
C(a)	MGI:3655080 (Map3k12)
C(b)	MGI:3655083 (Mapk8) MGI:3655084 (Mapk9)
C(c)	MGI:3655085 (Mapk8) MGI:3655086 (Mapk9)
C(e)	MGI:3655087 (Map2)
C(f)	MGI:3655089 (Vim)
A(a) p-JNK	MGI:3655072 (Mapk8) MGI:3655078 (Mapk9)
A(b) p-p38	MGI:3655075 (Mapk14)
A(c) p-ERK	MGI:3655070 (Mapk1) MGI:3655079 (Mapk3)