Gene Expression Image Detail

Reference

Figure

ID

J:63683 Bouchard M, et al., Development. 2000 Sep;127(17):3703-13

3

MGI:2149644

Image

Caption

(A) RNAse protection analysis. Total RNA isolated from kidneys of wild-type (+/+), heterozygous (5ki/+) and homozygous mutant (5ki/5ki) embryos at E18.5 was analyzed with individual or combined riboprobes for Pax2 (2), Pax5 (5) and/or lacZ (Z). (B) Quantitation of the RNase protection data. The level of each mRNA is shown as a percentage relative to the sum of all three mRNAs transcribed from the wild-type and targeted Pax2 alleles. (C) Western blot analysis of whole cell extracts (10 and 30 ug of protein) of kidneys from wild-type (+/+) and homozygous mutant (5ki/5ki) embryos at E18.5 was analyzed with polyclonal anti-paired domain and monoclonal anti-TBP antibodies. Reference Pax2b and Pax5 proteins were synthesized by couple in vitro transcription/translation.

Copyright

This image is from Bouchard M, Development 2000 Sep;127(17):3703-13 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A Pax5	MGI:2149811 (Pax5)
A Pax2	MGI:2149813 (Pax2)
C Pax2	MGI:2149815 (Pax2)
C Pax5	MGI:2149816 (Pax5)
C Pax8	MGI:2149817 (Pax8)