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Gene Expression Data
Assay Details
Assay
Reference: J:340701 Lee SJ, et al., Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway. Cell Death Differ. 2021 May;28(5):1441-1454
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8403140
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab13840)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1G: postnatal day 1 (more )
Note: Double labeled: red - Ddx4; green - Mast4 (assay MGI:8403135).
Structure Level Pattern Image Note
TS27: germ cell of testis Present Regionally restricted 1G inset , 1G Expression in cytoplam of germ cell, localized in outermost layer of seminiferous tubule.
TS27: seminiferous tubule Present Regionally restricted 1G inset , 1G Expression in cytoplam of germ cell, localized in outermost layer of seminiferous tubule.
Specimen 1K: postnatal week 3 (more )
Note: Double labeled: red - Ddx4; green - Mast4 (assay MGI:8403135).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Widespread 1K , 1K inset Expression in spermatogonia located throughout seminiferous tubules.
TS28: spermatogonium Present Widespread 1K , 1K inset Expression in spermatogonia located throughout seminiferous tubules.
Specimen 1R: postnatal week 6 (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present 1R inset , 1R
Specimen 1S: postnatal week 6; Mast4em1Sjkm/Mast4em1Sjkm (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 1S inset , 1S Expression in normal seminiferous tubules but not in Sertoli cell-only (SCO) tubules.
Specimen S1D: embryonic day 15.5 (more )
Note: Double labeled: red - Ddx4; green - Mast4 (assay MGI:8403135).
Structure Level Pattern Image Note
TS23: germ cell of testis Present Regionally restricted S1D , S1D inset Expression in spermatogonia and spermatocytes.
Specimen S1H: postnatal week 1 (more )
Note: Double labeled: red - Ddx4; green - Mast4 (assay MGI:8403135).
Structure Level Pattern Image Note
TS28: spermatocyte Present S1H inset , S1H
TS28: spermatogonium Present S1H inset , S1H
Specimen S1L: postnatal week 13 (more )
Note: Double labeled: red - Ddx4; green - Mast4 (assay MGI:8403135).
Structure Level Pattern Image Note
TS28: spermatocyte Present S1L , S1L inset
TS28: spermatogonium Present S1L , S1L inset
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
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