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Gene Expression Data
Assay Details
Assay
Reference: J:272622 Mochizuki K, et al., SETDB1 is essential for mouse primordial germ cell fate determination by ensuring BMP signaling. Development. 2018 Dec 3;145(23):dev164160
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8329537
Gene symbol: Smad5
Gene name: SMAD family member 5
Antibody: anti-Phospho-Smad1(Ser463/465)/ Smad5(Ser463/465)/ Smad9(Ser465/467) (D5B10) (#13820)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A WT: embryonic day 7.5 (more )
Note: Triple labeled: green - Smad5 and Smad1 and Smad9; red - Prdm1 (assay MGI:8329534); cyan - T (assay MGI:8329535).
Structure Level Pattern Image Note
TS10: epiblast Present Regionally restricted 3A Merge WT , 3A p-SMAD1/5/8 WT Expression was detected in the posterior-proximal epiblast.
TS10: embryo mesoderm Present 3A Merge WT , 3A p-SMAD1/5/8 WT
Specimen 3A Setdb1 cKO: embryonic day 7.5; Edil3Tg(Sox2-cre)1Amc/0, Setdb1tm1.1Yshk/Setdb1tm1.1Yshk (more )
Note: Conditional mutant. Triple labeled: green - Smad5 and Smad1 and Smad9; red - Prdm1 (assay MGI:8329534); cyan - T (assay MGI:8329535).
Structure Level Pattern Image Note
TS10: epiblast Weak Regionally restricted 3A Merge Setdb1 cKO , 3A p-SMAD1/5/8 Setdb1 cKO Expression in the posterior-proximal epiblast was reduced in the Setdb1 cKO.
TS10: embryo mesoderm Weak Regionally restricted 3A p-SMAD1/5/8 Setdb1 cKO , 3A Merge Setdb1 cKO Reduced expression was also observed in the adjacent mesodermal cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/15/2026
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