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Gene Expression Data
Assay Details
Assay
Reference: J:272622 Mochizuki K, et al., SETDB1 is essential for mouse primordial germ cell fate determination by ensuring BMP signaling. Development. 2018 Dec 3;145(23):dev164160
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8329534
Gene symbol: Prdm1
Gene name: PR domain containing 1, with ZNF domain
Antibody: anti-Blimp-1 (6D3; sc-47732)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 3A WT: embryonic day 7.5 (more )
Note: Triple labeled: red - Prdm1; green - Smad1 (assay MGI:8329536) and Smad5 (assay MGI:8329537) and Smad9 (assay MGI:8329538); cyan - T (assay MGI:8329535).
Structure Level Pattern Image Note
TS10: primordial germ cell Present 3A Merge WT , 3A BLIMP1 WT
Specimen 3A Setdb1 cKO: embryonic day 7.5; Edil3Tg(Sox2-cre)1Amc/0, Setdb1tm1.1Yshk/Setdb1tm1.1Yshk (more )
Note: Conditional mutant. Triple labeled: red - Prdm1; green - Smad1 (assay MGI:8329536) and Smad5 (assay MGI:8329537) and Smad9 (assay MGI:8329538); cyan - T (assay MGI:8329535).
Structure Level Pattern Image Note
TS10: primordial germ cell Weak 3A BLIMP1 Setdb1 cKO , 3A Merge Setdb1 cKO A significantly smaller number of Prdm1 (BLIMP1)-positive nascent primordial germ cells were observed in Setdb1 cKO embryos than in wild-type (WT) embryos.
Specimen 5A Setdb1 Hetero: embryonic day 7.5; Setdb1tm1.1Yshk/Setdb1+ (more )
Note: Double labeled: red - Prdm1; green - Utf1 (assay MGI:8329540).
Structure Level Pattern Image Note
TS10: primordial germ cell Present 5A BLIMP1 Setdb1 Hetero , 5A Merge Setdb1 Hetero
Specimen 5A Setdb1 cKO: embryonic day 7.5; Edil3Tg(Sox2-cre)1Amc/0, Setdb1tm1.1Yshk/Setdb1tm1.1Yshk (more )
Note: Conditional mutant. Double labeled: red - Prdm1; green - Utf1 (assay MGI:8329540).
Structure Level Pattern Image Note
TS10: primordial germ cell Weak 5A BLIMP1 Setdb1 cKO , 5A Merge Setdb1 cKO
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/15/2026
MGI 6.29 		The Jackson Laboratory