Antibody:	PCDH9 Polyclonal antibody (25090-1-AP)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Strong		4a Pcdh9 , 4a merge	A strong, coarse granular signal is present on the basolateral membranes.
TS23: ureteric tip	Strong		4a Pcdh9 , 4a merge	A strong, coarse granular signal is present on the basolateral membranes.
TS23: cortical renal tubule	Present	Regionally restricted	4a Pcdh9 , 4a merge	In the proximal convoluted tubule, expression appears as granular staining on the apical membrane, whereas in the remaining developing convoluted tubules, weaker diffuse staining is observed along the membranes.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Strong		4b merge , 4b Pcdh9	A strong, coarse granular signal is present on the basolateral membranes.
TS23: ureteric tip	Strong		4b merge , 4b Pcdh9	A strong, coarse granular signal is present on the basolateral membranes.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Strong		4c Pcdh9 , 4c merge	A strong, coarse granular signal is present on the basolateral membranes.
TS23: ureteric tip	Strong		4c Pcdh9 , 4c merge	A strong, coarse granular signal is present on the basolateral membranes.
TS23: cortical renal tubule	Present	Regionally restricted	4c merge , 4c Pcdh9	In the proximal convoluted tubule, expression appears as granular staining on the apical membrane, whereas in the remaining developing convoluted tubules, weaker diffuse staining is observed along the membranes.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Strong		4d Pcdh9 , 4d merge	A strong, coarse granular signal is present on the basolateral membranes.
TS23: ureteric tip	Strong		4d Pcdh9 , 4d merge	A strong, coarse granular signal is present on the basolateral membranes.
TS23: cortical renal tubule	Present	Regionally restricted	4d Pcdh9 , 4d merge	In the proximal convoluted tubule, expression appears as granular staining on the apical membrane, whereas in the remaining developing convoluted tubules, weaker diffuse staining is observed along the membranes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: visceral epithelium of mature renal corpuscle		Present	Diffuse	5a Pcdh9 , 5a merge	Diffuse cytoplasmic staining was observed in the visceral layer of the Bowman's capsule.
TS28: renal connecting tubule		Strong	Punctate	5a Pcdh9 , 5a merge	Expressed in the apical membranes.
TS28: distal convoluted tubule		Present	Diffuse	5a merge , 5a Pcdh9	Diffuse cytoplasmic staining, most intense basolaterally.
TS28: renal small blood vessel	blood vessel endothelial cell	Present	Punctate	5a Pcdh9 , 5a merge
TS28: renal proximal convoluted tubule		Present		5a Pcdh9 , 5a merge	Randomly distributed.

Structure	Level	Pattern	Image	Note
TS28: medullary collecting duct	Strong		5b Pcdh9 , 5b merge	Strong granular staining was detected on the apical membranes.
TS28: loop of Henle thick limb	Present	Diffuse	5b merge , 5b Pcdh9
TS28: loop of Henle thin limb	Absent		5b Pcdh9 , 5b merge

Structure	Level	Pattern	Image	Note
TS28: renal connecting tubule	Strong	Punctate	5c merge , 5c Pcdh9	Larger accumulations of signals in the form of granules were observed on the apical membranes.
TS28: distal convoluted tubule	Present	Diffuse	5c Pcdh9 , 5c merge	Expressed in a similar spatial pattern with markedly stronger signals, compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS28: medullary collecting duct	Strong		5d Pcdh9 , 5d merge	Expressed as a granular signal on the apical membranes with markedly stronger signals, compared with the wild-type.
TS28: loop of Henle thick limb	Present	Diffuse	5d merge , 5d Pcdh9	Expressed in a similar spatial pattern with markedly stronger signals, compared with the wild-type.
TS28: loop of Henle thin limb	Absent		5d Pcdh9 , 5d merge

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present			The area percentage occupied by the positive signal was consistent, with no significant differences observed in the mutant.

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Present
TS28: renal medulla	Present

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Present			A statistically significant increase in expression was observed, compared with the wild-type.
TS28: renal medulla	Present			A statistically significant increase in expression was observed, compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Present		S3_Pcdh9 , S3_merge middle