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Gene Expression Data
Assay Details
Assay
Reference: J:364871 Casado-Navarro R, et al., Expression atlas of Dmrt genes across sex and development in the mouse brain: functional insights from the olfactory system. Biol Sex Differ. 2026;17(1):44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8310125
Gene symbol: Th
Gene name: tyrosine hydroxylase
Antibody: anti-TH (rabbit; P40101-150)
Detection system: Secondary antibody
Assay notes: The authors reported using two Th antibodies but did not specify which was used to obtain these data; it is possible MGI:5447990 was used to obtain them.
Results
Specimen 1R: embryonic day 12.5 (more )
Note: Sagittal view. Double labeled: green - Th; red - Dmrta1 (assay MGI:8310121).
Structure Cell Type Level Pattern Image Note
TS20: midbrain-hindbrain junction central nervous system neuron Present 1R

Specimen 1S: embryonic day 14.5 (more )
Note: Coronal view. Double labeled: green - Th; red - Dmrta1 (assay MGI:8310121).
Structure Cell Type Level Pattern Image Note
TS22: midbrain central nervous system neuron Present 1S

Specimen 1T: embryonic day 13.5 (more )
Note: Coronal view. Double labeled: green - Th; blue - Dmrta2 (assay MGI:8310122).
Structure Cell Type Level Pattern Image Note
TS21: midbrain central nervous system neuron Present 1T

Specimen 1U: embryonic day 13.5 (more )
Note: Coronal view. Double labeled: green - Th; red - Dmrtb1 (assay MGI:8310123).
Structure Cell Type Level Pattern Image Note
TS21: midbrain central nervous system neuron Present 1U

Specimen 2H: embryonic day 18.5 (more )
Note: Double labeled: green - Th; red - Dmrtb1 (assay MGI:8310123).
Structure Cell Type Level Pattern Image Note
TS26: ventral tegmental area central nervous system neuron Present 2H , 2h'
TS26: substantia nigra central nervous system neuron Present 2h'' , 2H

Specimen 4b: embryonic day 18.5 (more )
Note: Coronal view. Double labeled: white - Th; green - Dmrta2 (assay MGI:8310122).
Structure Cell Type Level Pattern Image Note
TS26: ventral tegmental area central nervous system neuron Present 4b

Specimen 8A WT Female: embryonic day 18.5 (more )
Note: Subjected to the iDISCO+ protocol. Double labeled: magenta - Th; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS26: main olfactory bulb glomerular layer olfactory bulb interneuron Present 8A WT

Specimen 8A Dmrt5-/- Female: embryonic day 18.5; Dmrta2tm1Ejbd/Dmrta2tm1Ejbd (more )
Note: Subjected to the iDISCO+ protocol. Double labeled: magenta - Th; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS26: main olfactory bulb glomerular layer olfactory bulb interneuron Present 8A Dmrt5-/- The volume of Th+ neurons seen in mutant females is comparable to what is seen in wild type. However, the density of these neurons increased significantly in the mutant females.

Specimen 8B WT Male: embryonic day 18.5 (more )
Note: Subjected to the iDISCO+ protocol. Double labeled: magenta - Th; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS26: main olfactory bulb glomerular layer olfactory bulb interneuron Present 8B WT

Specimen 8B Dmrt5-/- Male: embryonic day 18.5; Dmrta2tm1Ejbd/Dmrta2tm1Ejbd (more )
Note: Subjected to the iDISCO+ protocol. Double labeled: magenta - Th; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS26: main olfactory bulb glomerular layer olfactory bulb interneuron Present 8B Dmrt5-/- The volume and density of Th+ neurons seen in mutant males is comparable to what is seen in wild type.

Specimen 8G: embryonic day 18.5 (more )
Note: Wild type.Subjected to the iDISCO+ protocol. Double labeled: magenta - Th; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS26: neocortex Ambiguous 8Gg' , 8G In wild type no expressing cells are found at the border of the neocortex and the piriform cortex .(Other areas of the neocortex were not described.)
TS26: piriform cortex neuron of the forebrain Present Single cells 8Gg'' , 8G In wild type the number of expressing cells is comparable in males and females.

Specimen 8H: embryonic day 18.5; Dmrta2tm1Ejbd/Dmrta2tm1Ejbd (more )
Note: Subjected to the iDISCO+ protocol. Double labeled: magenta - Th; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS26: neocortex cerebral cortex neuron Present Single cells 8Hh' , 8H Ectopic expressing cells are seen in the mutant, at the border of the neocortex and the piriform cortex.
TS26: piriform cortex neuron of the forebrain Present Single cells 8Hh'' , 8H The number of expressing cells is significantly increased in the mutant; this number is significantly increased in females relative to males.

Specimen 8I: embryonic day 18.5 (more )
Note: Wild type. Subjected to the iDISCO+ protocol. Double labeled: magenta - Th; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS26: neocortex Ambiguous 8Ii' , 8I In wild type no expressing cells are found at the border of the neocortex and the piriform cortex .(Other areas of the neocortex were not described.)
TS26: piriform cortex neuron of the forebrain Present Single cells 8Ii'' , 8I In wild type the number of expressing cells is comparable in males and females.

Specimen 8J: embryonic day 18.5; Dmrta2tm1Ejbd/Dmrta2tm1Ejbd (more )
Note: Subjected to the iDISCO+ protocol. Double labeled: magenta - Th; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS26: neocortex cerebral cortex neuron Present Single cells 8J , 8Jj' Ectopic expressing cells are seen in the mutant, at the border of the neocortex and the piriform cortex.
TS26: piriform cortex neuron of the forebrain Present Single cells 8Jj'' , 8J The number of expressing cells is significantly increased in the mutant; this number is significantly increased in females relative to males.

Specimen S5A: embryonic day 18.5 (more )
Note: Wild type. Subjected to the iDISCO+ protocol. Double labeled: green - Th; red - Pax6 (assay MGI:8315530).
Structure Cell Type Level Pattern Image Note
TS26: piriform cortex neuron of the forebrain Present Single cells S5A , S5Aa''

Specimen S5B: embryonic day 18.5; Dmrta2tm1Ejbd/Dmrta2tm1Ejbd (more )
Note: Subjected to the iDISCO+ protocol. Double labeled: green - Th; red - Pax6 (assay MGI:8315530).
Structure Cell Type Level Pattern Image Note
TS26: neocortex cerebral cortex neuron Present Single cells S5Bb' , S5B Ectopic expressing cells are seen in the mutant, at the border of the neocortex and the piriform cortex; they are Pax6+.
TS26: piriform cortex neuron of the forebrain Present Single cells S5B , S5Bb''

Specimen S5C: embryonic day 18.5 (more )
Note: Wild type. Subjected to the iDISCO+ protocol. Double labeled: green - Th; red - Pax6 (assay MGI:8315530).
Structure Cell Type Level Pattern Image Note
TS26: piriform cortex neuron of the forebrain Present Single cells S5C , S5Cc''

Specimen S5D: embryonic day 18.5; Dmrta2tm1Ejbd/Dmrta2tm1Ejbd (more )
Note: Subjected to the iDISCO+ protocol. Double labeled: green - Th; red - Pax6 (assay MGI:8315530).
Structure Cell Type Level Pattern Image Note
TS26: neocortex cerebral cortex neuron Present Single cells S5Dd' , S5D Ectopic expressing cells are seen in the mutant, at the border of the neocortex and the piriform cortex; they are Pax6+.
TS26: piriform cortex neuron of the forebrain Present Single cells S5D , S5Dd''


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last database update
10/06/2026
MGI 6.29
The Jackson Laboratory