Antibody:	anti-Calbindin D28k (CB-38)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cortical interneuron	Present	Non-Uniform	5a''	A severe reduction in Calb-positive interneurons density was observed within the patches of reduced Rbfox3 (NeuN)-positive labeling in efnA2 KO.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cortical interneuron	Present	Non-Uniform	5b	The majority of the immunopositive cells populated the supragranular layers 2/3 and fewer cells in the infragranular layers 5/6.
TS28: cortical layer II	cortical interneuron	Present		5b
TS28: cortical layer III	cortical interneuron	Present		5b
TS28: cortical layer V	cortical interneuron	Weak		5b
TS28: cortical layer VI	cortical interneuron	Weak		5b

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cortical interneuron	Weak	Non-Uniform	5b'	Calbindin-positive interneurons were present in the areas of the neocortex excluding patches, exhibiting a distribution profile comparable to the wild type control with the majority of the cells populating the supragranular layers 2/3 and fewer cells in the infragranular layers 5/6. However, the density of calbindin-positive cells throughout the entire neocortex, excluding the abnormal patches, was significantly reduced in the mutant.
TS28: cortical layer II	cortical interneuron	Weak		5b'
TS28: cortical layer III	cortical interneuron	Weak		5b'
TS28: cortical layer V	cortical interneuron	Weak		5b'
TS28: cortical layer VI	cortical interneuron	Weak		5b'