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Gene Expression Data
Assay Details
Assay
Reference: J:115581 Tian J, et al., Loss of Nkx2.8 deregulates progenitor cells in the large airways and leads to dysplasia. Cancer Res. 2006 Nov 1;66(21):10399-407
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:8282808
Gene symbol: Nkx2-9
Gene name: NK2 homeobox 9
Reporter: lacZ
Antibody: anti-B-galactosidase (100-4136)
Detection system: Secondary antibody coupled to alkaline phosphatase
Results
Specimen 6C Keratin/LacZ: postnatal month 6; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Note: Double labeled: black - Nkx2-9; red - Keratin.
Structure Level Pattern Image Note
TS28: bronchus epithelium Present Non-Uniform 6C-1 Strongly LacZ-positive cells on the basement membrane were negative for keratin, but some keratinized cells were weakly LacZ positive.
Specimen 6C betaTubulin IV/LacZ: postnatal month 6; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Note: Double labeled: black - Nkx2-9 reporter; red - Tubb4a (assay MGI:8282809) and Tubb4b (assay MGI:8283286).
Structure Cell Type Level Pattern Image Note
TS28: bronchus epithelium multiciliated epithelial cell of the bronchus Present 6C-2 Some of mature ciliated cells also showed weak LacZ-positive staining.
Specimen 6C CC10/LacZ: postnatal month 6; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Note: Double labeled: black - Nkx2-9 reporter; red - Scgb1a1 (assay MGI:8282810).
Structure Cell Type Level Pattern Image Note
TS28: bronchus epithelium club cell Present 6C-3 Some of Clara cells also showed weak LacZ-positive staining.
Specimen 6C p63/LacZ +/-: postnatal month 6; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Note: Double labeled: black - Nkx2-9 reporter; red - Trp63 (assay MGI:8282811).
Structure Cell Type Level Pattern Image Note
TS28: bronchus epithelium basal cell of epithelium of bronchus Present 6C-4 Strong staining of LacZ and Trp63 colocalized in most basal cells near the lung hilum. Other basal cells, however, were positive only for p63, particularly in more peripheral bronchi.
Specimen 6C p63/LacZ -/-: postnatal month 6; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Note: Double labeled: black - Nkx2-9 reporter; red - Trp63 (assay MGI:8282811).
Structure Cell Type Level Pattern Image Note
TS28: bronchus epithelium basal cell of epithelium of bronchus Present Scattered 6C-5 The homozygous had numerous LacZ+Trp63- basal cells, and in many areas these single positive cells had displaced the LacZ+Trp63+ upward into a parabasal location.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
MGI 6.29 		The Jackson Laboratory