About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:272613 Koutelou E, et al., USP22 controls multiple signaling pathways that are essential for vasculature formation in the mouse placenta. Development. 2019 Feb 22;146(4):dev174037
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8278822
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-Pecam (MEC 13.3)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2D WT: embryonic day 11.0 (more )
Note: Double labeled: green - Pecam1; red - phalloidin.
Structure Level Pattern Image Note
TS18: endocardial lining Present 2D WT PHA/PECAM1/DAPI , 2D WT PECAM1/DAPI
Specimen 2D Usp22-/-: embryonic day 11.0; Usp22Gt(RRS377)Byg/Usp22Gt(RRS377)Byg (more )
Note: Double labeled: green - Pecam1; red - phalloidin.
Structure Level Pattern Image Note
TS18: endocardial lining Present 2D Usp22-/- PHA/PECAM1/DAPI , 2D Usp22-/- PECAM1/DAPI Normal formation of all four heart chambers (right and left ventricle, right and left atrium) as well as the interventricular septum was observed by immunostaining.
Specimen S8 WT: embryonic day 10.0 (more )
Note: Double labeled: green - Pecam1; red - phalloidin.
Structure Level Pattern Image Note
TS16: endocardial lining Present S8_ WT PHA/PECAM1/DAPI , S8_ WT PHA/PECAM1/DAPI box , S8_ WT PECAM1
Specimen S8 Usp22-/-: embryonic day 10.0; Usp22Gt(RRS377)Byg/Usp22Gt(RRS377)Byg (more )
Note: Double labeled: green - Pecam1; red - phalloidin.
Structure Level Pattern Image Note
TS16: endocardial lining Present S8_ Usp22-/- PHA/PECAM1/DAPI , S8_ Usp22-/- PECAM1 , S8_ Usp22-/- PHA/PECAM1/DAPI box Normal staining of the endocardium was observed in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
10/06/2026
MGI 6.29 		The Jackson Laboratory