Gene Expression Data
Assay Details
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Assay
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Reference:
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J:290119
Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:8272323
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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anti-Islet-1 (clone 40.3A4)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
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Results
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Specimen
S2c WT:
embryonic day 13.5
(more  )
Specimen
S2c WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S2c Nolz1-/-:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Zfp503tm1.1Bpb/Zfp503tm1.1Bpb
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Present |
Regionally restricted |
S2c Nolz1-/- bottom
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Several aberrantly located ISL1+ positive cells were observed within the Nolz1<-/-> globus pallidus.
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Analysis Tools
