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Gene Expression Data
Assay Details
Assay
Reference: J:290119 Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8270717
Gene symbol: Th
Gene name: tyrosine hydroxylase
Antibody: anti-TH (rabbit; P40101-150)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present). Two Th antibodies were listed MGI:6159135 and MGI:4460872; the one used in this assay was not specified.
Results
Specimen S1c WT (with cCasp3): embryonic day 18.5 (more )
Note: Double labeled: green - Th; red - Casp3 (assay MGI:8270720).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c WT TH cCASP3
Specimen S1c Nolz1bGal/bGal (with cCasp3): embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - Th; red - Casp3 (assay MGI:8270720).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c Nolz1bgal/bgal TH cCASP3
Specimen S1c WT (with NolZ1/bGal): embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: green - Th; red - Zfp503 reporter (assay MGI:8270491).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c WT NOLZ1/bGAL TH
Specimen S1c Nolz1bGal/bGal (with NolZ1/bGal): embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - Th; red - Zfp503 reporter (assay MGI:8270491).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c Nolz1bgal/bgal NOLZ1/bGAL TH
Specimen S2d WT: embryonic day 18.5 (more )
Note: Double labeled: green - Th; red - Casp3 (assay MGI:8270720).
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S2d WT top , S2d WT bottom Expression was detected in dopaminergic axons.
Specimen S2d Nolz1-/-: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - Th; red - Casp3 (assay MGI:8270720).
Structure Level Pattern Image Note
TS26: brain Present Regionally restricted S2d Nolz1-/- bottom , S2d Nolz1-/- top Dopaminergic axons are misguided in Nolz1-/- mutant embryos, a large proportion of immunolabelled axons cross the midline in the hypothalamus and terminate rostral of the striatum in the mutant embryos.
Specimen S2d Foxg1IresCre;Nolz1fl/fl: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: green - Th; red - Casp3 (assay MGI:8270720).
Structure Level Pattern Image Note
TS26: corpus striatum Weak Regionally restricted S2d Foxg1-IresCre;Nolz1fl/fl bottom , S2d Foxg1-IresCre;Nolz1fl/fl top There was a strong reduction in striatal innervation by dopaminergic terminals in Foxg1IresCre;Nolz1<fl/fl> embryos, although to a lesser extent compared with Nolz1-/- mutant embryos.
Specimen S6o: embryonic day 18.5 (more )
Note: Double labeled: green - Th; red - Casp3 (assay MGI:8270720).
Structure Level Pattern Image Note
TS26: diencephalon Present S6o
Specimen S6p: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: green - Th; red - Casp3 (assay MGI:8270720).
Structure Level Pattern Image Note
TS26: diencephalon Present S6p
Specimen S6r: embryonic day 18.5 (more )
Note: Double labeled: green - Th; red - Casp3 (assay MGI:8270720).
Structure Level Pattern Image Note
TS26: diencephalon Present S6r
Specimen S6s: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: green - Th; red - Casp3 (assay MGI:8270720).
Structure Level Pattern Image Note
TS26: diencephalon Present S6s
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
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