Ddx4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:361642 He Y, et al., HFM1 is essential for the germ cell intercellular bridge transport in primordial follicle formation in mice. Cell Mol Life Sci. 2024 Dec 27;82(1):28
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8263048
Gene symbol:	Ddx4
Gene name:	DEAD box helicase 4

Antibody:	Anti-DDX4/MVH (ab13840)
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	Two Ddx4 antibodies were described, but the one used in this assay was not detailed. anti-DDX4 (ab27591) MGI:6690812 may have been used in this assay.

Results

1d WT E15.5 ©

Specimen 1d WT E15.5: embryonic day 15.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS23: ovary	female germ cell	Present		1d WT E15.5

1d WT E17.5 ©

Specimen 1d WT E17.5: embryonic day 17.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS25: ovary	female germ cell	Present		1d WT E17.5

1d WT P1 ©

Specimen 1d WT P1: postnatal day 1 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		1d WT P1

1d WT P3 ©

Specimen 1d WT P3: postnatal day 3 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		1d WT P3

1d KO E15.5 ©

Specimen 1d KO E15.5: embryonic day 15.5; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: ovary	female germ cell	Present		1d KO E15.5	Quantily of germ cells comparable to wild type at E15.5.

1d KO E17.5 ©

Specimen 1d KO E17.5: embryonic day 17.5; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: ovary	female germ cell	Weak		1d KO E17.5	Sharp decrease in number of expressing cells at E17.5.

1d KO P1 ©

Specimen 1d KO P1: postnatal day 1; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Weak		1d KO P1	Number of expressing cells continued to decline.

1d KO P3 ©

Specimen 1d KO P3: postnatal day 3; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Trace		1d KO P3	Loss of almost all germ cells at P3.

1i WT E17.5 left ©

1i WT E17.5 right ©

Specimen 1i WT E17.5 DDX4: embryonic day 17.5 (more

)
Note: Double labeled: green - Ddx4; red - Casp3 (assay MGI:8263050).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: ovary	female germ cell	Present		1i WT E17.5 right , 1i WT E17.5 left

1i WT P1 left ©

1i WT P1 right ©

Specimen 1i WT P1 DDX4: postnatal day 1 (more

)
Note: Double labeled: green - Ddx4; red - Casp3 (assay MGI:8263050).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		1i WT P1 left , 1i WT P1 right

1i WT P3 left ©

1i WT P3 right ©

Specimen 1i WT P3 DDX4: postnatal day 3 (more

)
Note: Double labeled: green - Ddx4; red - Casp3 (assay MGI:8263050).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		1i WT P3 right , 1i WT P3 left

1i KO E17.5 left ©

1i KO E17.5 right ©

Specimen 1i KO E17.5 DDX4: embryonic day 17.5; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Casp3 (assay MGI:8263050).

Structure	Level	Pattern	Image	Note
TS25: ovary	Weak		1i KO E17.5 left , 1i KO E17.5 right

1i KO P1 left ©

1i KO P1 right ©

Specimen 1i KO P1 DDX4: postnatal day 1; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Casp3 (assay MGI:8263050).

Structure	Level	Pattern	Image	Note
TS27: ovary	Trace		1i KO P1 left , 1i KO P1 right

1i KO P3 left ©

1i KO P3 right ©

Specimen 1i KO P3 DDX4: postnatal day 3; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Casp3 (assay MGI:8263050).

Structure	Level	Pattern	Image	Note
TS27: ovary	Trace		1i KO P3 right , 1i KO P3 left

2b WT E17.5 left ©

2b WT E17.5 right ©

Specimen 2b WT E17.5 DDX4: embryonic day 17.5 (more

)
Note: Double labeled: green - Ddx4; red - Golga2 (assay MGI:8263051).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: ovary	female germ cell	Present		2b WT E17.5 right , 2b WT E17.5 left

2b WT P1 left ©

2b WT P1 right ©

Specimen 2b WT P1 DDX4: postnatal day 1 (more

)
Note: Double labeled: green - Ddx4; red - Golga2 (assay MGI:8263051).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		2b WT P1 right , 2b WT P1 left

2b WT P3 left ©

2b WT P3 right ©

Specimen 2b WT P3 DDX4: postnatal day 3 (more

)
Note: Double labeled: green - Ddx4; red - Golga2 (assay MGI:8263051).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		2b WT P3 right , 2b WT P3 left

2b KO E17.5 left ©

2b KO E17.5 right ©

Specimen 2b KO E17.5 DDX4: embryonic day 17.5; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Golga2 (assay MGI:8263051).

Structure	Level	Pattern	Image	Note
TS25: ovary	Present		2b KO E17.5 left , 2b KO E17.5 right

2b KO P1 left ©

2b KO P1 right ©

Specimen 2b KO P1 DDX4: postnatal day 1; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Golga2 (assay MGI:8263051).

Structure	Level	Pattern	Image	Note
TS27: ovary	Weak		2b KO P1 left , 2b KO P1 right

2b KO P3 left ©

2b KO P3 right ©

Specimen 2b KO P3 DDX4: postnatal day 3; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Golga2 (assay MGI:8263051).

Structure	Level	Pattern	Image	Note
TS27: ovary	Trace		2b KO P3 left , 2b KO P3 right

3b WT E17.5 left ©

3b WT E17.5 right ©

Specimen 3b WT E17.5 DDX4: embryonic day 17.5 (more

)
Note: Double labeled: green - Ddx4; red - Pard6a (assay MGI:8263054).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: ovary	female germ cell	Present		3b WT E17.5 right , 3b WT E17.5 left

3b WT P1 left ©

3b WT P1 right ©

Specimen 3b WT P1 DDX4: postnatal day 1 (more

)
Note: Double labeled: green - Ddx4; red - Pard6a (assay MGI:8263054).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		3b WT P1 right , 3b WT P1 left

3b WT P3 left ©

3b WT P3 right ©

Specimen 3b WT P3 DDX4: postnatal day 3 (more

)
Note: Double labeled: green - Ddx4; red - Pard6a (assay MGI:8263054).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		3b WT P3 right , 3b WT P3 left

Specimen 3b KO E17.5 DDX4: embryonic day 17.5; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Pard6a (assay MGI:8263054).

Structure	Level	Pattern	Image	Note
TS25: ovary	Present		3b KO E17.5 left , 3b KO E17.5 right

Specimen 3b KO P1 DDX4: postnatal day 1; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Pard6a (assay MGI:8263054).

Structure	Level	Pattern	Image	Note
TS27: ovary	Weak		3b KO P1 left , 3b KO P1 right

Specimen 3b KO P3 DDX4: postnatal day 3; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Pard6a (assay MGI:8263054).

Structure	Level	Pattern	Image	Note
TS27: ovary	Trace		3b KO P3 left , 3b KO P3 right

Specimen 3c WT E17.5 DDX4: embryonic day 17.5 (more

)
Note: Double labeled: green - Ddx4; red - Foxl2 (assay MGI:8263055).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: ovary	female germ cell	Present		3c WT E17.5 left , 3c WT E17.5 right

Specimen 3c WT P1 DDX4: postnatal day 1 (more

)
Note: Double labeled: green - Ddx4; red - Foxl2 (assay MGI:8263055).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		3c WT P1 right , 3c WT P1 left

Specimen 3c WT P3 DDX4: postnatal day 3 (more

)
Note: Double labeled: green - Ddx4; red - Foxl2 (assay MGI:8263055).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ovary	female germ cell	Present		3c WT P3 left , 3c WT P3 right

Specimen 3c KO E17.5 DDX4: embryonic day 17.5; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Foxl2 (assay MGI:8263055).

Structure	Level	Pattern	Image	Note
TS25: ovary	Present		3c KO E17.5 right , 3c KO E17.5 left

Specimen 3c KO P1 DDX4: postnatal day 1; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. l Double labeled: green - Ddx4; red - Foxl2 (assay MGI:8263055).

Structure	Level	Pattern	Image	Note
TS27: ovary	Trace		3c KO P1 left , 3c KO P1 right

Specimen 3c KO P3 DDX4: postnatal day 3; Hfm1^em1Gpt/Hfm1^em1Gpt, Igs2^{em6(Gdf9-icre)Gpt}/Igs2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ddx4; red - Foxl2 (assay MGI:8263055).

Structure	Level	Pattern	Image	Note
TS27: ovary	Trace		3c KO P3 left , 3c KO P3 right

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