Probe:	Ebf2 probe
Probe preparation:	labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	1A	Expression first detected in preplate and cortical hem.sion
TS17: cortical hem	Present		1A

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present		1B
TS20: cortical hem	Present		1B
TS20: lateral ventricle choroid plexus	Absent		1B
TS20: ventral pallium	Present	Regionally restricted	1B	Expression extended to piriform cortex, part of mantle zone of ventral pallium.

Structure	Level	Pattern	Image	Note
TS21: telencephalon septum	Present

Structure	Level	Pattern	Image	Note
TS21: cortical hem	Present		1C
TS21: cortical preplate	Present		1C
TS21: telencephalon marginal layer	Present		1C	Expression in marginal zone
TS21: cortical subplate	Present		1C
TS21: lateral ventricle choroid plexus	Absent		1C

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present		1E , 1F
TS20: cortical hem	Present		1F , 1E
TS20: lateral ventricle choroid plexus	Absent		1E , 1F

Structure	Level	Pattern	Image	Note
TS20: telencephalon septum	Present		1H

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1I	Expression in piriform cortex, not co-localized with Reln expression.

Structure	Level	Pattern	Image	Note
TS23: cortical hem	Weak	Regionally restricted	1J	Expression dowregulated in pallial regions from E15.5
TS23: telencephalon marginal layer	Weak	Regionally restricted	1J	Expression dowregulated in pallial regions from E15.5
TS23: amygdala	Present		1J	Expression persists in piriform cortex and subcortical nuclei.
TS23: piriform cortex	Present		1J	Expression persists in piriform cortex and subcortical nuclei.
TS23: lateral ventricle choroid plexus	Absent		1J

Structure	Level	Pattern	Image	Note
TS25: telencephalon marginal layer	Weak	Regionally restricted	1K	Expression dowregulated in palial regions from E15.5
TS25: amygdala	Present		1K	Expression persists in amygdala.

Structure	Level	Pattern	Image	Note
TS27: bed nucleus of stria terminalis	Present		1L	Expression only detected in zona incerta and bed nucleus of stria terminalis.
TS27: zona incerta	Present		1L	Expression only detected in zona incerta and bed nucleus of stria terminalis.

Structure	Level	Pattern	Image	Note
TS20: cortical hem	Present	Regionally restricted	5B	Expression restricted to subdomain in cortical hem.

Structure	Level	Pattern	Image	Note
TS20: cortical hem	Ambiguous		5B'

Structure	Level	Pattern	Image	Note
TS20: telencephalon septum	Present	Regionally restricted	5E	Expression in septum confined dorsally.

Structure	Level	Pattern	Image	Note
TS23: cortical hem	Ambiguous	Regionally restricted	5H	Expression downregulated in marginal zone and cortical hem.
TS23: telencephalon marginal layer	Ambiguous	Regionally restricted	5H	Expression downregulated in marginal zone and cortical hem.