Smad3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:196581 Gui T, et al., The loss of Trps1 suppresses ureteric bud branching because of the activation of TGF-beta signaling. Dev Biol. 2013 May 15;377(2):415-27
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8235424
Gene symbol:	Smad3
Gene name:	SMAD family member 3

Antibody:	anti-p-Smad3
Detection system:	Secondary antibody coupled to Cy3

Results

5F E14.5 WT ©

Specimen 5F E14.5 WT: embryonic day 14.5 (more

)
Note: Double labeled: red - Smad3; green - cytokeratin.

Structure	Level	Pattern	Image	Note
TS22: ureteric tip	Trace	Single cells	5F E14.5 WT
TS22: cap mesenchyme	Present	Regionally restricted	5F E14.5 WT	Expression in other cells, including cap mesenchyme and stromal cells.
TS22: mesenchyme of metanephros	Present	Regionally restricted	5F E14.5 WT	Expression in other cells, including cap mesenchyme and stromal cells.

5F E14.5 KO ©

Specimen 5F E14.5 KO: embryonic day 14.5; Trps1^tm1Ymur/Trps1^tm1Ymur (more

)
Note: Double labeled: red - Smad3; green - cytokeratin.

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Present	Regionally restricted	5F E14.5 KO	Expression levels increased compared to wild type. Expression readily detected in nuclei of ureteric bud cells, especially in tips.
TS22: ureteric tip	Strong		5F E14.5 KO

5F E16.5 WT ©

Specimen 5F E16.5 WT: embryonic day 16.5 (more

)
Note: Double labeled: red - Smad3; green - cytokeratin.

Structure	Level	Pattern	Image	Note
TS24: ureteric tip	Trace	Single cells	5F E16.5 WT	Only a few expressing cells in ureteric bud tips.
TS24: cap mesenchyme	Present	Regionally restricted	5F E16.5 WT	Expression in other cells, including cap mesenchyme and stromal cells.
TS24: mesenchyme of metanephros	Present	Regionally restricted	5F E16.5 WT	Expression in other cells, including cap mesenchyme and stromal cells.

5F E16.5 KO ©

Specimen 5F E16.5 KO: embryonic day 16.5; Trps1^tm1Ymur/Trps1^tm1Ymur (more

)
Note: Double labeled: red - Smad3; green - cytokeratin.

Structure	Level	Pattern	Image	Note
TS24: collecting duct	Present	Regionally restricted	5F E16.5 KO	Expression levels increased compared to wild type. Expression readily detected in nuclei of ureteric bud cells, especially in tips.
TS24: ureteric tip	Strong		5F E16.5 KO

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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