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Gene Expression Data
Assay Details
Assay
Reference: J:184585 Yamamoto M, et al., The transformation suppressor gene Reck is required for postaxial patterning in mouse forelimbs. Biol Open. 2012;1(5):458-466
Assay type: RNA in situ
MGI Accession ID: MGI:8213284
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 5B7: embryonic day 10.5; Recktm1.1Noda/Reck+ (more )
Note: right forelimb
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 5B7
Specimen 5B8: embryonic day 10.5; Recktm1.1Noda/Recktm2.2Noda (more )
Note: right forelimb
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 5B8 Expression was downregulated in mutant samples.
Specimen 5C7: embryonic day 11.5; Recktm1.1Noda/Reck+ (more )
Note: right forelimb
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 5C7
Specimen 5C8: embryonic day 11.5; Recktm1.1Noda/Recktm2.2Noda (more )
Note: right forelimb
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 5C8 Expression was downregulated in mutant samples. Expression was often truncated on both termini (anterior and posterior) and sometimes discontinuous near the posterior end.
Specimen 5D3: embryonic day 10.5; Recktm2.1Noda/Recktm2.2Noda (more )
Note: Double labeled: blue - Fgf8 and Lmx1b.
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 5D3
Specimen 5D4: embryonic day 10.5; Recktm2.1Noda/Recktm2.2Noda, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: blue - Fgf8 and Lmx1b.
Structure Level Pattern Image Note
TS17: forelimb bud Present 5D4 Similar as in the control.
Specimen 5D5: embryonic day 11.5; Recktm2.1Noda/Recktm2.2Noda (more )
Note: Double labeled: blue - Fgf8 and Lmx1b.
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 5D5
Specimen 5D6: embryonic day 11.5; Recktm2.1Noda/Recktm2.2Noda, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: blue - Fgf8 and Lmx1b.
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 5D6 Reduced expression in the mutant.
Specimen S6B1: embryonic day 10.5; Recktm1.1Noda/Reck+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present S6B1
TS17: hindlimb bud apical ectodermal ridge Present S6B1
Specimen S6B2: embryonic day 10.5; Recktm1.1Noda/Reck+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present S6B2
TS17: hindlimb bud apical ectodermal ridge Present S6B2
Specimen S6B3: embryonic day 10.5; Recktm1.1Noda/Recktm2.2Noda (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present S6B3 Expression was downregulated in mutant samples. Expression was often truncated on both termini (anterior and posterior).
TS17: hindlimb bud apical ectodermal ridge Present S6B3 Similar as in the control.
Specimen S6B4: embryonic day 10.5; Recktm1.1Noda/Recktm2.2Noda (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present S6B4 Expression was downregulated in mutant samples. Expression was often truncated on both termini (anterior and posterior) and sometimes discontinuous near the posterior end.
TS17: hindlimb bud apical ectodermal ridge Present S6B4 Similar as in the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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07/22/2025
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