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Gene Expression Data
Assay Details
Assay
Reference: J:184585 Yamamoto M, et al., The transformation suppressor gene Reck is required for postaxial patterning in mouse forelimbs. Biol Open. 2012;1(5):458-466
Assay type: RNA in situ
MGI Accession ID: MGI:8213283
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 5B1: embryonic day 10.5; Recktm1.1Noda/Reck+ (more )
Note: right forelimb
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted 5B1 Expressed in the zone of polarizing activity in the distal-posterior region.
Specimen 5B2: embryonic day 10.5; Recktm1.1Noda/Recktm2.2Noda (more )
Note: right forelimb
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present 5B2 Expression was detectable but somewhat weaker than the control.
Specimen 5C1: embryonic day 11.5; Recktm1.1Noda/Reck+ (more )
Note: right forelimb
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 5C1 Expressed in the zone of polarizing activity in the distal-posterior region.
Specimen 5C2: embryonic day 11.5; Recktm1.1Noda/Recktm2.2Noda (more )
Note: right forelimb
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Weak 5C2 The area normally positive was partially missing in the mutants.
Specimen 5D1: embryonic day 10.5; Recktm2.1Noda/Recktm2.2Noda (more )
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted 5D1 Expressed in the zone of polarizing activity in the distal-posterior region.
Specimen 5D2: embryonic day 10.5; Recktm2.1Noda/Recktm2.2Noda, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present 5D2 Reduced expression in the mutant.
Specimen S6A5: embryonic day 10.5; Recktm1.1Noda/Reck+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted S6A5 Expressed in the zone of polarizing activity in the distal-posterior region.
Specimen S6A6: embryonic day 10.5; Recktm1.1Noda/Recktm2.2Noda (more )
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present S6A6 Expression was detectable but somewhat weaker than the control.
Specimen S7B1/3: embryonic day 11.5 (more )
Note: Color-concerted and overlaid: magenta - Shh; green - Reck (assay MGI:8213289).
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted S7B1 , S7B3 Expressed in the zone of polarizing activity in the distal-posterior region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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07/22/2025
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