Gene Expression Data
Assay Details
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Assay
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Reference:
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J:256095
Huang WC, et al., Diverse Non-genetic, Allele-Specific Expression Effects Shape Genetic Architecture at the Cellular Level in the Mammalian Brain. Neuron. 2017 Mar 8;93(5):1094-1109.e7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:8211133
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Gene symbol:
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Map1b
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Gene name:
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microtubule-associated protein 1B
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Probe:
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Mm-Mtap1b-intron2 (439841)
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Probe preparation:
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Antisense DNA
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Visualized with:
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Chromogenic
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Assay notes:
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Nascent RNA in situ hybridization analysis.
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Results
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Specimen
3D:
postnatal adult
(more  )
Specimen
3D:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dorsal raphe nucleus |
Present |
Regionally restricted |
3D
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Map1b exhibits biallelic expression in 66% of positive cells.
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| TS28: periaqueductal grey |
Present |
Regionally restricted |
3D
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Map1b exhibits biallelic expression in 66% of positive cells.
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Specimen
3E:
postnatal adult
(more )
Note: Double labeled: blue - Map1b; red - Syn2 (assay MGI:8211136).
Specimen
3E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Map1b; red - Syn2 (assay MGI:8211136).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: dorsal raphe nucleus |
central nervous system neuron |
Present |
Regionally restricted |
3E
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Co-labeling for Map1b+Syn2 revealed subpopulations of neurons in the midbrain that exhibit biallelic Syn2 expression but monoallelic expression for Map1b.
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Analysis Tools
