Probe:	Dmrt2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: telencephalon septum	Present	Regionally restricted	1B	Expression is seen in the subpallial septum.
TS20: preoptic area	Present		1B
TS20: lateral ventricle choroid plexus	Absent		1B

Structure	Level	Pattern	Image	Note
TS20: preoptic area	Present		1B'

Structure	Level	Pattern	Image	Note
TS20: nasal epithelium	Strong	Regionally restricted	1V Dmrt2	Expression is seen in the ventral respiratory epithelium.
TS20: vomeronasal organ	Strong	Scattered	1V Dmrt2

Structure	Level	Pattern	Image	Note
TS20: preoptic area	Present	Regionally restricted		Expression is seen in the mantle zone.
TS20: hypothalamus	Present	Regionally restricted		Although expression is seen elsewhere in the hypothalamus (the preoptic area) no expression is seen in the terminal hypothalamus paraventricular and subparaventricular areas; peduncular hypothalamus paraventricular area; or the retromammillary area.
TS20: mammillary area	Absent
TS20: telencephalon roof plate	Absent
TS20: lateral ventricle choroid plexus	Absent
TS20: subpallium	Present	Regionally restricted		Expression is seen in the subpallial septum. No expression is seen in the paraseptal subpallium; diagonal domain; pallidum; or the pallial-subpallial boundary.
TS20: corpus striatum	Absent
TS20: pallium	Absent			No expression is seen in the pallial-subpallial boundary.
TS20: ventral pallium	Absent
TS20: lateral pallium	Absent
TS20: neocortex	Absent			No expression is seen in the dorsal pallium.
TS20: allocortex	Absent			No expression is seen in the medial pallium.
TS20: telencephalon septum	Present	Regionally restricted		Expression is seen in the subpallial septum ventricular zone. No expression is seen in the pallial septum.
TS20: prosomere 3	Absent			No expression is seen in alar prethalamus, including prethalamic eminence and reticular complex, or in prethalamic tegmentum, including ventral tegmental area (A10), p3 part; substantia nigra (A9), p3 part; and posterior hypothalamic area, p3 part.
TS20: zona incerta	Absent
TS20: prosomere 2	Absent			No expression is seen in alar thalmus, including dorsal tier, or thalamic tegmentum, including ventral tegmental area (A10), p2 part; substantia nigra (A9), p2 part; and posterior hypothalamic area, p2 part.
TS20: habenula	Absent
TS20: prosomere 1	Absent			No expression is seen in alar plate or pretectal tegmentum, including ventral tegmental area (A10), p1 part; substantia nigra (A9), p1 part; and red nucleus, parvicelluar part.
TS20: mesomere 1	Absent			No expression is seen in collicular midbrain tectum, including superior and inferior colliculi and reticular formation, or in m1 midbrain tegmentum, including ventral tegmental area, m1 part; substantia nigra, m1 part; red nucleus, magnocellular part; and oculomotor nucleus.
TS20: mesomere 2	Absent			No expression is seen in preisthmic midbrain tectum or preisthmic tegmentum.
TS20: midbrain-hindbrain junction	Absent			No expression is seen in the isthmus, including the cerebellar vermis.
TS20: metencephalon	Absent			No expression is seen in the prepontine hindbrain (rhombomeres 1 and 2). This includes: A4, A6 and A7 noradrenergic cell groups and median raphe nucleus (B5).
TS20: pons	Absent			No expression is seen in the pontine hindbrain (rhombomeres 3 and 4). This includes: A5 noradrenergic cell group, r4 part.
TS20: medulla oblongata	Absent			No expression is seen in the pontomedullary hindbrain (rhombomeres 5 and 6) or the medullary hindbrain (rhombomeres 7-11). This includes: A5 noradrenergic cell group, r5 part; raphe magnus nucleus (B3); gigantocellular reticular area; and B1 and B2 serotonergic cell groups.
TS20: spinal cord	Absent
TS20: olfactory epithelium	Absent			No expression is seen in main olfactory epithelium.
TS20: nasal epithelium	Present			Expression is seen in respiratory epithelium.
TS20: vomeronasal organ	Present

Structure	Level	Pattern	Image	Note
TS28: orbital area	Present		3A first	Expression is seen in the orbital cortex.

Structure	Level	Pattern	Image	Note
TS28: prelimbic area	Present		3A second	Expression is seen in the prelimbic cortex.
TS28: orbital area	Present		3A second	Expression is seen in the orbital cortex.
TS28: endopiriform nucleus	Present		3A second	Expression is seen in the endopiriform cortex.
TS28: infralimbic area	Present		3A second

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Present		3A third
TS28: claustrum	Present		3A third
TS28: insular cortex	Present		3A third	Expression is seen in the agranular insular area.
TS28: endopiriform nucleus	Present		3A third	Expression is seen in the endopiriform cortex.
TS28: lateral septal nucleus	Present	Regionally restricted	3A third	Expression is seen in the caudal part.

Structure	Level	Pattern	Image	Note
TS28: retrosplenial cortex	Present		3A fourth
TS28: hippocampus CA1	Present		3A fourth
TS28: dentate gyrus	Present		3A fourth
TS28: claustrum	Present		3A fourth
TS28: endopiriform nucleus	Present	Scattered	3A fourth	Expression is seen in the endopiriform cortex.
TS28: basolateral amygdaloid nucleus	Present		3A fourth

Structure	Level	Pattern	Image	Note
TS28: retrosplenial cortex	Present		3A fifth
TS28: subiculum	Present		3A fifth
TS28: endopiriform nucleus	Present	Scattered	3A fifth	Expression is seen in the endopiriform cortex.
TS28: basomedial amygdaloid nucleus	Present		3A fifth
TS28: piriform cortex	Present		3A fifth
TS28: cortical amygdalar area	Present		3A fifth

Structure	Level	Pattern	Image	Note
TS28: subiculum	Present		3A sixth	Expression is seen in the pyramidal layer.

Structure	Level	Pattern	Image	Note
TS28: periaqueductal grey	Present	Scattered	3A seventh

Structure	Level	Pattern	Image	Note
TS26: periaqueductal grey	Present	Scattered	3B	Expression levels are more robust than at P7.

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Present		3C
TS28: dentate gyrus	Present		3C
TS28: hippocampal formation	Present		3C
TS28: hippocampus CA1	Present		3C
TS28: neocortex	Present		3C

Structure	Level	Pattern	Image	Note
TS28: preoptic area	Present		3E Male in situ

Structure	Level	Pattern	Image	Note
TS28: preoptic area	Present		3E Female in situ

Structure	Level	Pattern	Image	Note
TS22: brain	Present		ISH_Dmrt2_E14_Female

Structure	Level	Pattern	Image	Note
TS22: brain	Present		ISH_Dmrt2_E14_Male

Structure	Level	Pattern	Image	Note
TS26: neocortex region	Present		S3_E18.5 Female lower , S3_E18.5 Female upper	Expression is seen in the orbital cortex; prelimbic area; infralimbic area; and the cingulate cortex.
TS26: olfactory cortex	Present		S3_E18.5 Female lower , S3_E18.5 Female upper	Expression is seen in the endopiriform cortex.
TS26: insular cortex	Present		S3_E18.5 Female upper , S3_E18.5 Female lower	Expression is seen in the agranular insular area.
TS26: retrosplenial cortex	Present		S3_E18.5 Female lower , S3_E18.5 Female upper
TS26: piriform cortex	Present		S3_E18.5 Female lower , S3_E18.5 Female upper
TS26: telencephalon septum	Present		S3_E18.5 Female upper , S3_E18.5 Female lower	Expression is seen in the lateral septal nucleus.
TS26: subpallium	Present		S3_E18.5 Female lower , S3_E18.5 Female upper	Expression is seen in the cortical amygdalar area.
TS26: amygdala	Present		S3_E18.5 Female upper , S3_E18.5 Female lower	Expression is seen in the basolateral amygdaloid nucleus.
TS26: hippocampus CA1	Present		S3_E18.5 Female upper , S3_E18.5 Female lower
TS26: dentate gyrus	Present		S3_E18.5 Female upper , S3_E18.5 Female lower
TS26: hippocampal formation	Present		S3_E18.5 Female lower , S3_E18.5 Female upper	Expression is seen in the subiculum.
TS26: basal ganglia	Present		S3_E18.5 Female lower , S3_E18.5 Female upper	Expression is seen in the claustrum.
TS26: periaqueductal grey	Present		S3_E18.5 Female lower , S3_E18.5 Female upper

Structure	Level	Pattern	Image	Note
TS26: neocortex region	Present		S3_E18.5 Male upper , S3_E18.5 Male lower	Expression is seen in the orbital cortex; prelimbic area; infralimbic area; and the cingulate cortex.
TS26: olfactory cortex	Present		S3_E18.5 Male lower , S3_E18.5 Male upper	Expression is seen in the endopiriform cortex.
TS26: insular cortex	Present		S3_E18.5 Male upper , S3_E18.5 Male lower	Expression is seen in the agranular insular area.
TS26: retrosplenial cortex	Present		S3_E18.5 Male upper , S3_E18.5 Male lower
TS26: piriform cortex	Present		S3_E18.5 Male upper , S3_E18.5 Male lower
TS26: telencephalon septum	Present		S3_E18.5 Male lower , S3_E18.5 Male upper	Expression is seen in the lateral septal nucleus.
TS26: subpallium	Present		S3_E18.5 Male upper , S3_E18.5 Male lower	Expression is seen in the cortical amygdalar area.
TS26: amygdala	Present		S3_E18.5 Male upper , S3_E18.5 Male lower	Expression is seen in the basolateral amygdaloid nucleus.
TS26: hippocampus CA1	Present		S3_E18.5 Male lower , S3_E18.5 Male upper
TS26: dentate gyrus	Present		S3_E18.5 Male upper , S3_E18.5 Male lower
TS26: hippocampal formation	Present		S3_E18.5 Male upper , S3_E18.5 Male lower	Expression is seen in the subiculum.
TS26: basal ganglia	Present		S3_E18.5 Male lower , S3_E18.5 Male upper	Expression is seen in the claustrum.
TS26: periaqueductal grey	Present		S3_E18.5 Male lower , S3_E18.5 Male upper

Structure	Level	Pattern	Image	Note
TS28: orbital area	Present			Expression is seen in the orbital cortex.
TS28: prelimbic area	Present			Expression is seen in the prelimbic cortex.
TS28: endopiriform nucleus	Present			Expression is seen in the endopiriform cortex.
TS28: infralimbic area	Present
TS28: claustrum	Present
TS28: insular cortex	Present			Expression is seen in the agranular insular area.
TS28: lateral septal nucleus	Present
TS28: retrosplenial cortex	Present
TS28: basolateral amygdaloid nucleus	Present
TS28: subiculum	Present
TS28: basomedial amygdaloid nucleus	Present
TS28: piriform cortex	Present
TS28: cortical amygdalar area	Present
TS28: periaqueductal grey	Present
TS28: hippocampus CA1	Present