Gene Expression Data
Assay Details
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Assay
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Reference:
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J:306107
Niborski LL, et al., Hnf1b haploinsufficiency differentially affects developmental target genes in a new renal cysts and diabetes mouse model. Dis Model Mech. 2021 May 1;14(5):dmm047498
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:8187730
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Gene symbol:
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Slc12a1
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Gene name:
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solute carrier family 12, member 1
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Antibody:
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anti-NKCC2
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Assay notes:
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Embryos and postnatal kidneys up to 2 months of age were fixed with 60% ethanol/11% formaldehyde and 10% acetic acid.
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Results
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Specimen
5N/P:
postnatal day 0
(more  )
Note: Double labeled: green - Slc12a1; red - pan-cytokeratin.
Specimen
5N/P:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Slc12a1; red - pan-cytokeratin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: loop of Henle |
Present |
Regionally restricted |
5P
,
5N
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Apical expression in epithelium of thick ascending limb of loop of Henle.
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Specimen
5O/Q:
postnatal day 0; Hnf1btm2.1Sce/Hnf1b +
(more )
Note: Double labeled: green - Slc12a1; red - pan-cytokeratin.
Specimen
5O/Q:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Hnf1btm2.1Sce/Hnf1b+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Slc12a1; red - pan-cytokeratin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: loop of Henle |
Weak |
Regionally restricted |
5O
,
5Q
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Decreased expression in cystic medullar loops of Henle, in a proximal-distal gradient and disorganized.
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Specimen
S5C:
embryonic day 17.5
(more )
Specimen
S5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
S5C':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Hnf1btm2.1Sce/Hnf1b+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: metanephros |
Present |
Regionally restricted |
S5C'
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Expression predominantly in medullar-cortical cystic structures and tubular dilations.
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Specimen
S5H:
postnatal day 0
(more )
Specimen
S5H:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
S5H':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Hnf1btm2.1Sce/Hnf1b+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: metanephros |
Present |
Regionally restricted |
S5H'
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Expression predominantly in medullar-cortical cystic structures and tubular dilations.
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Analysis Tools