Sema5b RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:334513 Yan K, et al., TrkB-dependent EphrinA reverse signaling regulates callosal axon fasciculate growth downstream of Neurod2/6. Cereb Cortex. 2023 Feb 20;33(5):1752-1767
Assay type:	RNA in situ
MGI Accession ID:	MGI:8187398
Gene symbol:	Sema5b
Gene name:	sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B

Probe:	Sema5b probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen S1 Control: embryonic day 16.5; Neurod2^tm1Mhsb/Neurod2⁺, Neurod6^tm1(cre)Kan/Neurod6^tm1(cre)Kan (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Present		S1_Control Sema5B (E16.5)
TS24: ganglionic eminence	Present	Regionally restricted	S1_Control Sema5B (E16.5)	Expression was detected in ganglionic eminence epithelium.

Specimen S1 DKO: embryonic day 16.5; Neurod2^tm1Mhsb/Neurod2^tm1Mhsb, Neurod6^tm1(cre)Kan/Neurod6^tm1(cre)Kan (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Present		S1_DKO Sema5B (E16.5)	Expression was not significantly different in Neurod2/6-deficient mice compared with control.
TS24: ganglionic eminence	Present		S1_DKO Sema5B (E16.5)	Expression was not significantly different in Neurod2/6-deficient mice compared with control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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