Probe:	Dmrt6 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: mammillary area	Present
TS20: hypothalamus	Present		1F	Areas of expression include the retromammillary area (within the peduncular hypothalamus).
TS20: mesomere 1	Present	Widespread	1F	Expression is seen in the ventral (tegmentum) and dorsal (tectum) parts.
TS20: mesomere 2	Present	Widespread	1F	Expression is seen in the ventral (tegmentum) and dorsal (tectum) parts.
TS20: prosomere 1	Present	Widespread	1F	Expression is seen in the ventral (tegmentum) and dorsal (tectum) parts.
TS20: prosomere 2	Present	Widespread	1F	Expression is seen in the ventral (tegmentum) and dorsal (tectum) parts.
TS20: prosomere 3	Present	Widespread	1F	Expression is seen in the ventral (tegmentum) and dorsal (tectum) parts.
TS20: subpallium	Present		1F	Areas of expression include the central subpallium.
TS20: lateral ventricle choroid plexus	Absent		1F

Structure	Level	Pattern	Image	Note
TS20: subpallium	Present		1F''	Areas of expression include the subpallial amygdala.
TS20: lateral pallium	Absent		1F''
TS20: ventral pallium	Absent		1F''
TS20: corpus striatum	Present		1F''

Structure	Level	Pattern	Image	Note
TS20: allocortex	Present	Regionally restricted	1f , 1F'	Expression is seen in the medial pallium ventricular and mantle zones.
TS20: thalamus	Present		1f , 1F'
TS20: corpus striatum	Present		1F'
TS20: preoptic area	Present		1F'
TS20: subpallium	Present		1F'	Expression is seen in the pallidum and paraseptal subpallium.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present		1G first
TS20: prethalamus	Present		1G first	Expression is seen in the prethalamic eminence.
TS20: corpus striatum	Present		1G first
TS20: subpallium	Present		1G first	Expression is seen in the pallidum.
TS20: allocortex	Present		1G first	Expression is seen in the dentate gyrus.
TS20: epithalamus	Present		1G first	Expression in seen in the stria medullaris.

Structure	Level	Pattern	Image	Note
TS20: prosomere 1	Present		1G second
TS20: prosomere 2	Present		1G second
TS20: prosomere 3	Present		1G second
TS20: hypothalamus	Present		1G second	Expression is seen in the peduncular and terminal hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		1G third	Areas of expression include the substantia nigra (dopaminergic A9 cell group); ventral tegmental area (dopaminergic A10 cell group); and the red nucleus.
TS20: mammillary area	Present		1G third
TS20: hypothalamus	Present		1G third	Areas of expression include the retromammillary area.
TS20: hindbrain	Present		1G third

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		1G fourth	Areas of expression include the oculomotor nucleus.
TS20: metencephalon	Present		1G fourth	Expression is seen in the median raphe nucleus (serotonergic B5 cell group).

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		1G fifth	Areas of expression include the superior colliculus.
TS20: hindbrain	Present		1G fifth	Expression is seen in the A4-A7 noradrenergic cell groups.

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present		1G sixth	Expression is seen in the cerebellar vermis.
TS20: medulla oblongata	Present	Regionally restricted	1G sixth	Expression is seen in the B1-B3 serotonergic (5-HT) group.
TS20: midbrain	Present		1G sixth	Areas of expression include the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1H Dmrt6	Expression is seen in the prethalamic eminence mantle zone; it is excluded from the ventricular zone.
TS20: hypothalamus	Present	Regionally restricted	1H Dmrt6	Expression is seen in the peduncular and terminal paraventricular areas.
TS20: thalamus	Present		1H Dmrt6

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted	1L	Expression is seen in the raphe magnus nucleus (serotonergic B3 cell group) ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted	1M left	Expression is seen in the raphe magnus nucleus (serotonergic B3 cell group) ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted	1M right	Expression is seen in the raphe magnus nucleus (serotonergic B3 cell group) ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: preoptic area	Present			Expression is seen in the mantle zone.
TS20: hypothalamus	Present			Expression is seen in the mantle zone of the terminal hypothalamus paraventricular and subparaventricular areas; peduncular hypothalamus paraventricular area mantle zone; and the retromammillary area mantle zone.
TS20: mammillary area	Present
TS20: telencephalon roof plate	Absent
TS20: lateral ventricle choroid plexus	Absent
TS20: subpallium	Present	Regionally restricted		Expression is seen in the paraseptal subpallium mantle zone; diagonal domain mantle zone; and the pallidum mantle zone. No expression is seen in the pallial-subpallial boundary.
TS20: corpus striatum	Present			Expression is seen in the mantle zone.
TS20: pallium	Present	Regionally restricted		Although expression is seen elsewhere in the pallium, no expression is seen in the pallial-subpallial boundary.
TS20: ventral pallium	Absent
TS20: lateral pallium	Absent
TS20: neocortex	Absent			No expression is seen in the dorsal pallium.
TS20: allocortex	Present	Regionally restricted		Expression is seen in the medial pallium ventricular and mantle zones.
TS20: telencephalon septum	Present	Regionally restricted		Expression is seen in the subpallial septum mantle zone. No expression is seen in the pallial septum.
TS20: prosomere 3	Present			Expression is seen in alar prethalamus mantle zones, including prethalamic eminence and reticular complex, and in prethalamic tegmentum mantle zones, including ventral tegmental area (A10), p3 part; substantia nigra (A9), p3 part; and posterior hypothalamic area, p3 part.
TS20: zona incerta	Present			Expression is seen in the mantle zone.
TS20: prosomere 2	Present			Expression is seen in the alar thalmus mantle zone, including dorsal tier, and thalamic tegmentum mantle zone, including ventral tegmental area (A10), p2 part; substantia nigra (A9), p2 part; and posterior hypothalamic area, p2 part.
TS20: habenula	Present
TS20: prosomere 1	Present			Expression is seen in alar plate and pretectal tegmentum mantle zones, including ventral tegmental area (A10), p1 part; substantia nigra (A9), p1 part; and red nucleus, parvicelluar part.
TS20: mesomere 1	Present			Expression is seen in collicular midbrain tectum mantle zones, including superior and inferior colliculi and reticular formation, and in m1 midbrain tegmentum mantle zones, including ventral tegmental area, m1 part; substantia nigra, m1 part; red nucleus, magnocellular part; and oculomotor nucleus.
TS20: mesomere 2	Present			Expression is seen in the preisthmic midbrain tectum and preisthmic tegmentum mantle zones.
TS20: midbrain-hindbrain junction	Present			Expression is seen in the isthmus, including cerebellar vermis, mantle zone.
TS20: metencephalon	Present			Expression is seen in the prepontine hindbrain (rhombomeres 1 and 2). This includes: A4, A6 and A7 noradrenergic cell groups and median raphe nucleus (B5).
TS20: pons	Present			Expression is seen in the pontine hindbrain (rhombomeres 3 and 4). This includes: A5 noradrenergic cell group, r4 part.
TS20: medulla oblongata	Present	Regionally restricted		Expression is seen in the pontomedullary hindbrain (rhombomeres 5 and 6) and the medullary hindbrain (rhombomeres 7-11). This includes: A5 noradrenergic cell group, r5 part; raphe magnus nucleus (B3) ventricular zone; and B1 and B2 serotonergic cell group ventricular zones. There is no expression in the gigantocellular reticular area.
TS20: spinal cord	Present
TS20: olfactory epithelium	Absent			No expression is seen in main olfactory epithelium.
TS20: nasal epithelium	Absent			No expression is seen in respiratory epithelium.
TS20: vomeronasal organ	Absent

Structure	Level	Pattern	Image	Note
TS26: olfactory cortex	Present		2A	Expression is seen in the anterior olfactory area (ventral and dorsal parts).
TS26: accessory olfactory bulb	Present		2A

Structure	Level	Pattern	Image	Note
TS26: telencephalon septum	Present		2B	Expression is seen in the medial septal nucleus; lateral septal nucleus; vertical nucleus of the diagonal band; and horizontal nucleus of the diagonal band.
TS26: piriform cortex	Present		2B
TS26: subpallium	Present		2B	Expression is seen in the substantia innominate/basal nucleus.

Structure	Level	Pattern	Image	Note
TS26: paraventricular thalamic nucleus	Present		2C
TS26: anterior thalamic group	Present		2C	Expression is seen in the anterodorsal nucleus.
TS26: telencephalon septum	Present		2C	Expression is seen in the bed nucleus of the stria terminalis and the anterior commissure.
TS26: globus pallidus	Present		2C
TS26: paraventricular hypothalamic nucleus	Present		2C
TS26: lateral preoptic area	Present		2C	Expression is seen in the lateral preoptic nucleus.
TS26: medial preoptic area	Present		2C	Expression is seen in the medial preoptic nucleus.

Structure	Level	Pattern	Image	Note
TS26: dorsal lateral geniculate nucleus	Present		2D
TS26: geniculate thalamic group	Present		2D	Expression is seen in the geniculate leaflet.
TS26: lateral habenular nucleus	Present		2D
TS26: ventral lateral geniculate nucleus	Present		2D	Expression is seen in the pregeniculate nucleus.
TS26: ventral lateral thalamic nucleus	Strong		2D
TS26: ventral medial thalamic nucleus	Present		2D
TS26: nucleus reuniens	Strong		2D
TS26: zona incerta	Strong		2D
TS26: globus pallidus	Present		2D
TS26: supraoptic nucleus	Present		2D
TS26: paraventricular hypothalamic nucleus	Strong		2D
TS26: anterior hypothalamic nucleus	Present		2D

Structure	Level	Pattern	Image	Note
TS26: pretectum	Present		2E	Expression is seen in the anterior pretectal nucleus; medial pretectal nucleus; and olivary pretectal nucleus.
TS26: periaqueductal grey	Present		2E
TS26: thalamus	Present		2E	Expression is seen in the prosomere 2 tegmentum.
TS26: prethalamus	Present		2E	Expression is seen in the prosomere 3 tegmentum.
TS26: zona incerta	Present		2E
TS26: subthalamic nucleus	Present		2E
TS26: hypothalamic nucleus	Present		2E	Expression is seen in the ventromedial nucleus
TS26: dorsomedial hypothalamic nucleus	Present		2E
TS26: amygdala	Present		2E	Expression is seen in the medial and cortical amygdaloid area.

Structure	Level	Pattern	Image	Note
TS26: periaqueductal grey	Present		2F
TS26: pretectum	Present		2F	Expression is seen in the anterior pretectal nucleus
TS26: medial geniculate nucleus	Present		2F
TS26: zona incerta	Present		2F
TS26: mammillary area	Present		2F
TS26: hypothalamus	Present		2F	Areas of expression include the retromammillary area.
TS26: substantia nigra	Present		2F

Structure	Level	Pattern	Image	Note
TS26: periaqueductal grey	Present		2G
TS26: superior colliculus	Present		2G
TS26: red nucleus	Strong		2G
TS26: substantia nigra	Present		2G
TS26: ventral tegmental area	Present		2G
TS26: hindbrain	Present		2G

Structure	Level	Pattern	Image	Note
TS26: periaqueductal grey	Present		2I
TS26: superior colliculus	Present		2I
TS26: midbrain tegmentum	Present		2I	Expression is seen in the dorsal raphe nucleus.
TS26: brainstem nucleus	Present		2I	Expression is seen in the median raphe nucleus and pontine reticular nucleus.

Structure	Level	Pattern	Image	Note
TS26: periaqueductal grey	Present		2J
TS26: superior colliculus	Present		2J
TS26: brainstem nucleus	Present		2J	Expression is seen in the dorsal tegmental nucleus.

Structure	Level	Pattern	Image	Note
TS26: retina ganglion cell layer	Present		2K , 2k'

Structure	Level	Pattern	Image	Note
TS28: medial amygdaloid nucleus	Present		2L	The area of expression overlaps with that of Esr2.
TS28: cortical amygdaloid nucleus	Present		2L

Structure	Level	Pattern	Image	Note
TS28: triangular septal nucleus	Present		2O
TS28: bed nucleus of stria terminalis	Present		2O
TS28: nucleus of anterior commissure	Present		2O

Structure	Level	Pattern	Image	Note
TS28: paraventricular hypothalamic nucleus	Present		2P
TS28: supraoptic nucleus	Present		2P

Structure	Level	Pattern	Image	Note
TS28: zona incerta	Present		2Q
TS28: dorsomedial hypothalamic nucleus	Present		2Q

Structure	Level	Pattern	Image	Note
TS26: lateral preoptic area	Present
TS26: medial preoptic area	Present
TS26: supraoptic nucleus	Present
TS26: paraventricular hypothalamic nucleus	Present			Expression is seen both the terminal and peduncular hypothalamus paraventricular nuclei.
TS26: anterior hypothalamic nucleus	Present
TS26: mammillary area	Present
TS26: hypothalamic nucleus	Present			Expression is seen in the ventromedial nucleus.
TS26: telencephalon septum	Present			Expression is seen in the medial septal nucleus; lateral septal nucleus; triangular septal nucleus; vertical nucleus of the diagonal band; bed nucleus of the stria terminalis; and bed nucleus of the anterior commissure.
TS26: subpallium	Present			Expression is seen in the bed nucleus of the stria terminalis; substantia innominata; and horizontal nucleus of the diagonal band.
TS26: globus pallidus	Present
TS26: amygdala	Present	Regionally restricted		Expression is seen in the medial amygdala and cortical amygdala.
TS26: indusium griseum	Present
TS26: olfactory cortex	Present			Expression is seen in the taenia tecta; anterior olfactory area (ventral and dorsal parts); and navicular nucleus.
TS26: piriform cortex	Present
TS26: accessory olfactory bulb	Present
TS26: subthalamic nucleus	Present
TS26: dorsomedial hypothalamic nucleus	Present
TS26: hypothalamus	Present			Expression is seen in the retromammillary area.
TS26: prethalamus	Present			Expression is seen in the prethalamic eminence.
TS26: reticular thalamic nucleus	Present
TS26: zona incerta	Present
TS26: ventral lateral geniculate nucleus	Present			Expression is seen in the pregeniculate nucleus.
TS26: ventral tegmental area	Present			Expression is seen in the ventral tegmental area of the prethalamus, thalamus, pretectum, and midbrain tegmentum.
TS26: substantia nigra	Present			Expression is seen in the substantia nigra of the prethalamus, thalamus, pretectum, and midbrain tegmentum.
TS26: lateral habenular nucleus	Present
TS26: paraventricular thalamic nucleus	Present			Expression is seen in the anterior paraventricular nucleus.
TS26: anterior thalamic group	Present			Expression is seen in the anterodorsal nucleus.
TS26: dorsal lateral geniculate nucleus	Present
TS26: paratenial nucleus	Present
TS26: ventral lateral thalamic nucleus	Present
TS26: ventral medial thalamic nucleus	Present
TS26: nucleus reuniens	Present
TS26: geniculate thalamic group	Present			Expression is seen in the geniculate leaflet.
TS26: pretectum	Present	Regionally restricted		Expression is seen in the anterior pretectal nucleus; medial pretectal nucleus; and olivary pretectal nucleus.
TS26: periaqueductal grey	Present			Expression is seen in the periaqueductal gray of the pretectum alar plate, pretectum tegmentum, superior colliculus, inferior colliculus, and midbrain tectum.
TS26: red nucleus	Present			Expression is seen in the red nucleus of the pretectal tegmentum and midbrain tegmentum.
TS26: superior colliculus	Present
TS26: inferior colliculus	Present
TS26: tectum	Present			Expression is seen in the reticular formation and preisthmic midbrain tectum.
TS26: midbrain tegmentum	Present	Regionally restricted		Expression is seen in the dorsal raphe nucleus.
TS26: midbrain-hindbrain junction	Present
TS26: brainstem nucleus	Present	Regionally restricted		Expression is seen in the dorsal tegmental nucleus and the median raphe nucleus (r1 and r2 parts).
TS26: hindbrain	Present			Expression is seen in the pontine, pontomedullary, and medullary hindbrain.
TS26: spinal cord	Present

Structure	Level	Pattern	Image	Note
TS28: lateral preoptic area	Present
TS28: medial preoptic area	Absent
TS28: supraoptic nucleus	Absent
TS28: paraventricular hypothalamic nucleus	Absent			No expression is seen in either the terminal or peduncular hypothalamus paraventricular nuclei.
TS28: anterior hypothalamic nucleus	Absent
TS28: mammillary area	Absent
TS28: ventromedial hypothalamic nucleus	Present
TS28: medial septal nucleus	Present
TS28: lateral septal nucleus	Absent
TS28: triangular septal nucleus	Present
TS28: vertical nucleus of diagonal band	Absent
TS28: bed nucleus of stria terminalis	Present
TS28: substantia inominata	Absent
TS28: horizontal nucleus of diagonal band	Absent
TS28: globus pallidus	Absent
TS28: medial amygdaloid nucleus	Present
TS28: indusium griseum	Absent
TS28: taenia tecta	Absent
TS28: anterior olfactory nucleus	Present			Expression is seen in the anterior olfactory area, ventral part; it is not seen in the anterior olfactory area, dorsal part.
TS28: cortical amygdalar area	Present			Expression is seen in the cortical amygdala.
TS28: navicular nucleus	Absent
TS28: piriform cortex	Absent
TS28: accessory olfactory bulb	Absent
TS28: subthalamic nucleus	Present
TS28: dorsomedial hypothalamic nucleus	Present
TS28: retromammillary area	Absent
TS28: prethalamic eminence	Absent
TS28: reticular thalamic nucleus	Present
TS28: zona incerta	Present
TS28: ventral lateral geniculate nucleus	Absent			No expression is seen in the pregeniculate nucleus.
TS28: ventral tegmental area	Present	Regionally restricted		Expression is seen in the thalamic, pretectal, and midbrain ventral tegmental areas. No expression is seen in the prethalamic ventral tegmental area.
TS28: substantia nigra	Present	Regionally restricted		Expression is seen in the thalamic, pretectal, and midbrain substantia nigra. No expression is seen in the prethalamic substantia nigra.
TS28: lateral habenular nucleus	Absent
TS28: paraventricular thalamic nucleus	Absent			No expression is seen in the anterior paraventricular nucleus.
TS28: anterior dorsal thalamic nucleus	Absent
TS28: dorsal lateral geniculate nucleus	Present
TS28: paratenial nucleus	Absent
TS28: ventral lateral thalamic nucleus	Absent
TS28: ventral medial thalamic nucleus	Absent
TS28: nucleus reuniens	Present
TS28: geniculate leaflet	Absent
TS28: anterior pretectal nucleus	Present
TS28: medial pretectal nucleus	Absent
TS28: olivary pretectal nucleus	Absent
TS28: periaqueductal grey	Present	Regionally restricted		Expression is seen in the periaqueductal gray of the pretectum alar plate, superior colliculus, and inferior colliculus. No expression is seen in the periaqueductal gray of the pretectal tegmentum and midbrain tectum.
TS28: red nucleus	Present
TS28: superior colliculus	Present
TS28: inferior colliculus	Absent
TS28: midbrain reticular formation	Absent
TS28: tectum	Absent			No expression is seen in the preisthmic midbrain tectum.
TS28: midbrain-hindbrain junction	Present
TS28: dorsal raphe nucleus	Present
TS28: dorsal tegmental nucleus	Present
TS28: median raphe nucleus	Present			Expression is seen in the median raphe nucleus (r1 and r2 parts).
TS28: pons	Present			Expression is seen in the pontine hindbrain.
TS28: pontomedullary hindbrain	Present
TS28: medulla oblongata	Present			Expression is seen in the medullary hindbrain.
TS28: spinal cord	Present

Structure	Level	Pattern	Image	Note
TS28: lateral preoptic area	Absent
TS28: medial preoptic area	Absent
TS28: supraoptic nucleus	Present
TS28: paraventricular hypothalamic nucleus	Present	Regionally restricted		Expression is seen in the peduncular hypothalmus paraventricular nucleus. No expression is seen in the terminal hypothalmus paraventricular nucleus.
TS28: anterior hypothalamic nucleus	Absent
TS28: mammillary area	Absent
TS28: ventromedial hypothalamic nucleus	Present
TS28: medial septal nucleus	Absent
TS28: lateral septal nucleus	Absent
TS28: triangular septal nucleus	Present
TS28: vertical nucleus of diagonal band	Absent
TS28: bed nucleus of stria terminalis	Present
TS28: nucleus of anterior commissure	Present
TS28: substantia inominata	Absent
TS28: horizontal nucleus of diagonal band	Absent
TS28: globus pallidus	Absent
TS28: medial amygdaloid nucleus	Absent
TS28: indusium griseum	Absent
TS28: taenia tecta	Absent
TS28: cortical amygdalar area	Absent			No expression is seen in the cortical amygdala.
TS28: navicular nucleus	Absent
TS28: piriform cortex	Absent
TS28: accessory olfactory bulb	Absent
TS28: anterior olfactory nucleus	Absent			No expression is seen in the anterior olfactory area, dorsal part. (The ventral part was not examined.)
TS28: subthalamic nucleus	Absent
TS28: dorsomedial hypothalamic nucleus	Present
TS28: retromammillary area	Absent
TS28: prethalamic eminence	Absent
TS28: reticular thalamic nucleus	Absent
TS28: zona incerta	Present
TS28: ventral lateral geniculate nucleus	Absent			No expression is seen in the pregeniculate nucleus.
TS28: ventral tegmental area	Absent			No expression is seen in the prethalamic ventral tegmental area. (The thalamic, pretectal, and midbrain ventral tegmental areas were not examined.)
TS28: substantia nigra	Absent			No expression is seen in the prethalamic substantia nigra. (The thalamic, pretectal, and midbrain substantia nigra were not examined.)
TS28: lateral habenular nucleus	Absent
TS28: paraventricular thalamic nucleus	Absent			No expression is seen in the anterior paraventricular nucleus.
TS28: anterior dorsal thalamic nucleus	Absent
TS28: paratenial nucleus	Absent
TS28: ventral lateral thalamic nucleus	Absent
TS28: ventral medial thalamic nucleus	Absent
TS28: geniculate leaflet	Absent
TS28: medial pretectal nucleus	Absent
TS28: olivary pretectal nucleus	Absent
TS28: periaqueductal grey	Absent			No expression is seen in the periaqueductal gray of the pretectum alar plate, the pretectum, or the midbrain tectum.
TS28: inferior colliculus	Absent
TS28: midbrain reticular formation	Absent
TS28: tectum	Absent			No expression is seen in the preisthmic midbrain tectum.
TS28: dorsal tegmental nucleus	Absent
TS28: median raphe nucleus	Absent			No expression is seen in the median raphe nucleus, r1 part (The r2 part was not examined.)
TS28: pons	Absent			No expression is seen in the pontine hindbrain.

Structure	Level	Pattern	Image	Note
TS20: brain	Present		ISH_Dmrt6_E12_Female

Structure	Level	Pattern	Image	Note
TS20: brain	Present		ISH_Dmrt6_E12_Male

Structure	Level	Pattern	Image	Note
TS22: brain	Present		ISH_Dmrt6_E14_Female

Structure	Level	Pattern	Image	Note
TS22: brain	Present		ISH_Dmrt6_E14_Male

Structure	Level	Pattern	Image	Note
TS26: brain	Present		S2_E18.5 Female lower , S2_E18.5 Female upper

Structure	Level	Pattern	Image	Note
TS26: brain	Present		S2_E18.5 Male lower , S2_E18.5 Male upper

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S2_P7 Female

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S2_P7 Male