Probe:	Dmrt5 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1E	Areas of expression include the prethalamic eminence.
TS20: prosomere 1	Present	Regionally restricted	1E	Expression is seen in the in the ventral part (tegmentum).
TS20: prosomere 2	Present	Regionally restricted	1E	Expression is seen in the in the ventral part (tegmentum).
TS20: prosomere 3	Present	Regionally restricted	1E	Expression is seen in the in the ventral part (tegmentum).
TS20: mesomere 1	Present	Regionally restricted	1E	Expression is seen in the in the ventral part (tegmentum).
TS20: mesomere 2	Present	Regionally restricted	1E	Expression is seen in the in the ventral part (tegmentum).
TS20: pituitary gland	Present		1E
TS20: lateral ventricle choroid plexus	Strong		1E
TS20: hypothalamus	Present	Regionally restricted	1E	Expression is seen in the terminal paraventricular area.

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1E'	Expression is seen in the prethalamic eminence.
TS20: pallium	Present		1E'
TS20: lateral ventricle choroid plexus	Strong		1E'
TS20: telencephalon	Present	Regionally restricted	1E'	Expression is seen in the pallial-subpallial boundary.

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1H Dmrt5	Expression is seen in the prethalamic eminence ventricular and mantle zones.
TS20: hypothalamus	Present	Regionally restricted	1H Dmrt5	Expression is seen in the peduncular and terminal paraventricular areas.

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis	Present	Regionally restricted	1O	Expression is seen in the anterior and intermediate lobes of the pituitary gland.

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis	Present	Regionally restricted	1P	Expression is seen in the anterior and intermediate lobes of the pituitary gland.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		1V Dmrt5	Expression is seen in the main olfactory epithelium.
TS20: nasal epithelium	Present	Regionally restricted	1V Dmrt5	Expression is seen in the lateral and ventral respiratory epithelium.

Structure	Level	Pattern	Image	Note
TS20: preoptic area	Absent
TS20: hypothalamus	Present	Regionally restricted		Expression is seen in the terminal hypothalamus paraventricular area ventricular and mantle zones and peduncular hypothalamus paraventricular area ventricular zone. No expression is seen in the terminal hypothalamus subparaventricular area or the retromammillary area.
TS20: mammillary area	Absent
TS20: telencephalon roof plate	Present	Regionally restricted
TS20: lateral ventricle choroid plexus	Present
TS20: subpallium	Present	Regionally restricted		Expression is seen in the paraseptal subpallium and pallial-subpallial boundary ventricular zone. No expression is seen in the diagonal domain or the pallidum.
TS20: corpus striatum	Absent
TS20: pallium	Present	Regionally restricted		Expression is seen in the pallial-subpallial boundary ventricular zone.
TS20: ventral pallium	Present	Regionally restricted		Expression is seen in the ventricular zone
TS20: lateral pallium	Present	Regionally restricted		Expression is seen in the ventricular zone
TS20: neocortex	Present	Regionally restricted		Expression is seen in the dorsal pallium ventricular zone.
TS20: allocortex	Present	Regionally restricted		Expression is seen in the medial pallium ventricular and mantle zones.
TS20: telencephalon septum	Present	Regionally restricted		Expression is seen in the subpallial and pallial septum ventricular zones.
TS20: prosomere 3	Present	Regionally restricted		Expression is seen in prethalamic eminence ventricular zone and in prethalamic tegmentum ventricular zones, including ventral tegmental area (A10), p3 part; substantia nigra (A9), p3 part; and posterior hypothalamic area, p3 part ventricular zones. No expression is seen in alar prethalamus reticular complex.
TS20: zona incerta	Absent
TS20: prosomere 2	Present	Regionally restricted		Expression is seen in thalamic tegmentum, including ventral tegmental area (A10), p2 part; substantia nigra (A9), p2 part; and posterior hypothalamic area, p2 part ventricular zones. No expression is seen in alar thalmus, including dorsal tier.
TS20: habenula	Absent
TS20: prosomere 1	Present	Regionally restricted		Expression is seen in pretectal tegmentum, including ventral tegmental area (A10), p1 part; substantia nigra (A9), p1 part; and red nucleus, parvicelluar part ventricular zones. No expression is seen in alar plate.
TS20: mesomere 1	Present	Regionally restricted		Expression is seen in m1 midbrain tegmentum ventricular zones, including ventral tegmental area, m1 part; substantia nigra, m1 part; and red nucleus, magnocellular part. No expression is seen in collicular midbrain tectum, including superior and inferior colliculi and reticular formation, or m1 midbrain tegmentum oculomotor nucleus.
TS20: mesomere 2	Present	Regionally restricted		Expression is seen in preisthmic tegmentum ventricular zone. No expression is seen in preisthmic midbrain tectum.
TS20: midbrain-hindbrain junction	Absent			No expression is seen in the isthmus, including the cerebellar vermis.
TS20: metencephalon	Absent			No expression is seen in the prepontine hindbrain (rhombomeres 1 and 2). This includes: A4, A6 and A7 noradrenergic cell groups and median raphe nucleus (B5).
TS20: pons	Absent			No expression is seen in the pontine hindbrain (rhombomeres 3 and 4). This includes: A5 noradrenergic cell group, r4 part.
TS20: medulla oblongata	Absent			No expression is seen in the pontomedullary hindbrain (rhombomeres 5 and 6) or the medullary hindbrain (rhombomeres 7-11). This includes: A5 noradrenergic cell group, r5 part; raphe magnus nucleus (B3); gigantocellular reticular area; and B1 and B2 serotonergic cell groups.
TS20: spinal cord	Absent
TS20: olfactory epithelium	Present			Expression is seen in main olfactory epithelium.
TS20: nasal epithelium	Present			Expression is seen in respiratory epithelium.
TS20: vomeronasal organ	Present

Structure	Level	Pattern	Image	Note
TS26: olfactory epithelium	Present		4A	Expression is seen in the main olfactory epithelium.
TS26: subpallium	Present	Scattered	4A	Expression is seen in the substantia innominate.

Structure	Level	Pattern	Image	Note
TS26: lateral ventricle choroid plexus	Present		4a
TS26: hypothalamus	Present		4a	Expression is seen in the lateral hypothalamus.
TS26: subpallium	Present		4a	Expression is seen in the substantia innominate.
TS26: 3rd ventricle ependyma	Present		4a	Expression is seen in potential neurogenic niches.

Structure	Level	Pattern	Image	Note
TS26: preoptic area	Present	Scattered	4B
TS26: midbrain	Present		4B
TS26: diencephalon	Present		4B
TS26: 3rd ventricle ependyma	Present		4B	Expression is seen in potential neurogenic niches.

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present		4c	Expression is seen in the Edinger- Westphal nucleus.
TS26: red nucleus	Present		4c
TS26: cerebral aqueduct	Present		4c	Expression is seen in potential neurogenic niches.

Structure	Level	Pattern	Image	Note
TS26: adenohypophysis	Present	Regionally restricted	4d	Expression is seen in the marginal cell layer of the intermediate lobe and in a few cells of the anterior lobe.

Structure	Level	Pattern	Image	Note
TS26: hypothalamus	Present		4C	Expression is seen in the lateral hypothalamus.
TS26: olfactory tubercle	Present	Scattered	4C
TS26: cerebral aqueduct	Present		4C	Expression is seen in potential neurogenic niches.

Structure	Level	Pattern	Image	Note
TS26: cerebral aqueduct	Present	Regionally restricted	4e	Expression is seen in potential neurogenic niches.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present		4D
TS26: olfactory epithelium	Present	Widespread	4D	Expression is seen in the main olfactory epithelium.
TS26: olfactory tubercle	Present	Widespread	4D
TS26: rostral migratory stream	Present	Widespread	4D
TS26: Grueneberg ganglion	Present	Widespread	4g , 4D

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb prospective granule cell layer	Present		4f'
TS26: main olfactory bulb subependymal zone	Present		4f'	Expression is seen in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb prospective granule cell layer	Present		4f''
TS26: main olfactory bulb glomerular layer	Present		4f''
TS26: main olfactory bulb subependymal zone	Present		4f''	Expression is seen in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS26: neocortex	Absent

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		4E
TS28: main olfactory bulb granule cell layer	Present		4E
TS28: main olfactory bulb subependymal zone	Present		4E	Expression is seen in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present	Regionally restricted	4F , 4h	Expression is restricted to a few glomeruli in the ventrolateral zone; the bead-like pattern resembles the necklace glomeruli.

Structure	Level	Pattern	Image	Note
TS28: lateral hypothalamic area	Present		4G
TS28: substantia inominata	Present	Scattered	4G

Structure	Level	Pattern	Image	Note
TS28: ventral tegmental area	Present		4H

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present		4I	Expression is seen in the posterior hypothalamic area.
TS28: 3rd ventricle ependyma	Present		4I	Expression is seen in the ventricular zone of the third ventricle.

Structure	Level	Pattern	Image	Note
TS28: dorsal raphe nucleus	Present		4J

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle choroid plexus epithelium	Strong		4K

Structure	Level	Pattern	Image	Note
TS28: 3rd ventricle choroid plexus	Absent
TS28: 4th ventricle choroid plexus	Absent

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb subependymal zone	Present	Regionally restricted	5A	Expression is seen in the dorsal subventricular zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: olfactory epithelium		Strong		6B , 6b'	There is a strong signal in the basal layer, where olfactory neural stem cells are maintained.
TS28: olfactory epithelium	olfactory epithelial supporting cell	Present		6B , 6b'	Expression is seen in the sustentacular cells of the apical layer.
TS28: vomeronasal organ		Absent		6B , 6b''

Structure	Level	Pattern	Image	Note
TS22: nasal epithelium	Present		7A
TS22: olfactory epithelium	Present		7A

Structure	Level	Pattern	Image	Note
TS22: nasal epithelium	Absent		7A'
TS22: olfactory epithelium	Absent		7A'

Structure	Level	Pattern	Image	Note
TS20: brain	Present		ISH_Dmrt5_E12_Female

Structure	Level	Pattern	Image	Note
TS20: brain	Present		ISH_Dmrt5_E12_Male

Structure	Level	Pattern	Image	Note
TS22: brain	Present		ISH_Dmrt5_E14_Female

Structure	Level	Pattern	Image	Note
TS22: brain	Present		ISH_Dmrt5_E14_Male

Structure	Level	Pattern	Image	Note
TS26: brain	Present		S4_E18.5 coronal view Female lower , S4_E18.5 coronal view Female upper

Structure	Level	Pattern	Image	Note
TS26: brain	Present		S4_E18.5 coronal view Male upper , S4_E18.5 coronal view Male lower

Structure	Level	Pattern	Image	Note
TS26: brain	Present		S4_E18.5 sagittal view Female upper , S4_E18.5 sagittal view Female lower

Structure	Level	Pattern	Image	Note
TS26: brain	Present		S4_E18.5 sagittal view Male lower , S4_E18.5 sagittal view Male upper