Probe:	Dmrt4 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1D	Expression is seen in the prethalamic eminence.
TS20: mesomere 2	Present		1D
TS20: prosomere 2	Present		1D
TS20: lateral ventricle choroid plexus	Absent		1D

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1D'	Expression is seen in the prethalamic eminence.
TS20: pallium	Present		1D'
TS20: telencephalon	Present	Regionally restricted	1D'	Expression is seen in the pallial-subpallial boundary.

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1H Dmrt4	Expression is seen in the prethalamic eminence ventricular zone.
TS20: hypothalamus	Present	Regionally restricted	1H Dmrt4	Expression is seen in the terminal paraventricular area.

Structure	Level	Pattern	Image	Note
TS20: habenula	Present		1I

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present		1I'	Expression is seen in the tegmentum of the pre-isthmus and isthmus.

Structure	Level	Pattern	Image	Note
TS20: nasal epithelium	Strong	Regionally restricted	1V Dmrt4	Expression is seen in the lateral respiratory epithelium.
TS20: vomeronasal organ	Present	Widespread	1V Dmrt4

Structure	Level	Pattern	Image	Note
TS20: preoptic area	Absent
TS20: hypothalamus	Present	Regionally restricted		Expression is seen in the terminal hypothalamus paraventricular area ventricular zone. No expression is seen in the terminal hypothalamus subparaventricular area; peduncular hypothalamus paraventricular area; or the retromammillary area.
TS20: mammillary area	Absent
TS20: telencephalon roof plate	Absent
TS20: lateral ventricle choroid plexus	Absent
TS20: subpallium	Present	Regionally restricted		Expression is seen in the pallial-subpallial boundary ventricular zone. No expression is seen in the paraseptal subpallium; diagonal domain; or the pallidum.
TS20: corpus striatum	Absent
TS20: pallium	Present	Regionally restricted		Expression is seen in the pallial-subpallial boundary ventricular zone.
TS20: ventral pallium	Present	Regionally restricted		Expression is seen in the ventricular zone.
TS20: lateral pallium	Present	Regionally restricted		Expression is seen in the ventricular zone.
TS20: neocortex	Present	Regionally restricted		Expression is seen in the dorsal pallium ventricular zone.
TS20: allocortex	Present	Regionally restricted		Expression is seen in the medial pallium ventricular and mantle zones.
TS20: telencephalon septum	Present	Regionally restricted		Expression is seen in the pallial septum ventricular zone. No expression is seen in the subpallial septum.
TS20: prosomere 3	Present	Regionally restricted		Expression is seen in prethalamic eminence and reticular complex ventricular zones. No expression is seen in prethalamic tegmentum, including ventral tegmental area (A10), p3 part; substantia nigra (A9), p3 part; and posterior hypothalamic area, p3 part.
TS20: zona incerta	Absent
TS20: prosomere 2	Present	Regionally restricted		Expression is seen in the habenula. No expression is seen in alar thalmus dorsal tier or thalamic tegmentum, including ventral tegmental area (A10), p2 part; substantia nigra (A9), p2 part; and posterior hypothalamic area, p2 part.
TS20: habenula	Present
TS20: prosomere 1	Absent			No expression is seen in alar plate or pretectal tegmentum, including ventral tegmental area (A10), p1 part; substantia nigra (A9), p1 part; and red nucleus, parvicelluar part.
TS20: mesomere 1	Absent			No expression is seen in collicular midbrain tectum, including superior and inferior colliculi and reticular formation, or in m1 midbrain tegmentum, including ventral tegmental area, m1 part; substantia nigra, m1 part; red nucleus, magnocellular part; and oculomotor nucleus.
TS20: mesomere 2	Present	Regionally restricted		Expression is seen in preisthmic tegmentum mantle zone. No expression is seen in preisthmic midbrain tectum.
TS20: midbrain-hindbrain junction	Present	Regionally restricted		Expression is seen in the isthmus, including cerebellar vermis, ventricular and mantle zones.
TS20: metencephalon	Absent			No expression is seen in the prepontine hindbrain (rhombomeres 1 and 2). This includes: A4, A6 and A7 noradrenergic cell groups and median raphe nucleus (B5).
TS20: pons	Absent			No expression is seen in the pontine hindbrain (rhombomeres 3 and 4). This includes: A5 noradrenergic cell group, r4 part.
TS20: medulla oblongata	Absent			No expression is seen in the pontomedullary hindbrain (rhombomeres 5 and 6) or the medullary hindbrain (rhombomeres 7-11). This includes: A5 noradrenergic cell group, r5 part; raphe magnus nucleus (B3); gigantocellular reticular area; and B1 and B2 serotonergic cell groups.
TS20: spinal cord	Absent
TS20: olfactory epithelium	Absent			No expression is seen in main olfactory epithelium.
TS20: nasal epithelium	Present			Expression is seen in respiratory epithelium.
TS20: vomeronasal organ	Present

Structure	Level	Pattern	Image	Note
TS22: nasal epithelium	Weak	Regionally restricted	7C	There is dim expression in the lateral part of the respiratory epithelium.
TS22: olfactory epithelium	Absent		7C
TS22: vomeronasal organ	Present		7C	Expression fills up the entire apical layer and the marginal zone.

Structure	Level	Pattern	Image	Note
TS22: nasal epithelium	Present		7C'	In the mutant expression is unregulated in the respiratory epithelium of both sexes.
TS22: olfactory epithelium	Present		7C'	In the mutant there is ectopic expression in the main olfactory epithelium of both sexes.
TS22: vomeronasal organ	Present		7C'

Structure	Level	Pattern	Image	Note
TS20: brain	Present		ISH_Dmrt4_E12_Female

Structure	Level	Pattern	Image	Note
TS20: brain	Present		ISH_Dmrt4_E12_Male

Structure	Level	Pattern	Image	Note
TS22: brain	Present		ISH_Dmrt4_E14_Female

Structure	Level	Pattern	Image	Note
TS22: brain	Present		ISH_Dmrt4_E14_Male