Probe:	Dmrt3 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1C	Expression is seen in the prethalamic eminence.
TS20: lateral ventricle choroid plexus	Strong		1C

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1C'	Expression is seen in the prethalamic eminence.
TS20: lateral ventricle choroid plexus	Strong		1C'
TS20: pallium	Strong		1C'

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Regionally restricted	1H Dmrt3	Expression is seen in the prethalamic eminence ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted	1J	Expression is seen in the gigantocellular reticular area.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Ambiguous		1K left

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted	1K right	Expression is seen in the gigantocellular reticular area.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		1V Dmrt3	Expression is seen in the main olfactory epithelium.
TS20: nasal epithelium	Present	Regionally restricted	1V Dmrt3	Expression is seen in the lateral and ventral respiratory epithelium.
TS20: vomeronasal organ	Present	Widespread	1V Dmrt3

Structure	Level	Pattern	Image	Note
TS20: preoptic area	Absent
TS20: hypothalamus	Absent			No expression is seen in the terminal hypothalamus paraventricular and subparaventricular areas; peduncular hypothalamus paraventricular area; or the retromammillary area.
TS20: mammillary area	Absent
TS20: telencephalon roof plate	Absent
TS20: lateral ventricle choroid plexus	Present
TS20: subpallium	Absent			No expression is seen in the subpallium, including paraseptal subpallium; diagonal domain; pallidum; or the pallial-subpallial boundary.
TS20: corpus striatum	Absent
TS20: pallium	Present	Regionally restricted		Although expression is seen elsewhere in the pallium, no expression is seen in the pallial-subpallial boundary.
TS20: ventral pallium	Present	Regionally restricted		Expression is seen in the ventricular zone.
TS20: lateral pallium	Present	Regionally restricted		Expression is seen in the ventricular zone.
TS20: neocortex	Present	Regionally restricted		Expression is seen in the dorsal pallium ventricular and mantle zones.
TS20: allocortex	Present	Regionally restricted		Expression is seen in the medial pallium ventricular and mantle zones.
TS20: telencephalon septum	Absent			No expression is seen in the subpallial septum or pallial septum.
TS20: prosomere 3	Present	Regionally restricted		Expression is seen in prethalamic eminence ventricular zone. No expression is seen in other regions of the alar prethalamus, including reticular complex, or in prethalamic tegmentum, including ventral tegmental area (A10), p3 part; substantia nigra (A9), p3 part; and posterior hypothalamic area, p3 part.
TS20: zona incerta	Absent
TS20: prosomere 2	Absent			No expression is seen in alar thalmus, including dorsal tier, or thalamic tegmentum, including ventral tegmental area (A10), p2 part; substantia nigra (A9), p2 part; and posterior hypothalamic area, p2 part.
TS20: habenula	Absent
TS20: prosomere 1	Absent			No expression is seen in alar plate or pretectal tegmentum, including ventral tegmental area (A10), p1 part; substantia nigra (A9), p1 part; and red nucleus, parvicelluar part.
TS20: mesomere 1	Absent			No expression is seen in collicular midbrain tectum, including superior and inferior colliculi and reticular formation, or in m1 midbrain tegmentum, including ventral tegmental area, m1 part; substantia nigra, m1 part; red nucleus, magnocellular part; and oculomotor nucleus.
TS20: mesomere 2	Absent			No expression is seen in preisthmic midbrain tectum or preisthmic tegmentum.
TS20: midbrain-hindbrain junction	Absent			No expression is seen in the isthmus, including the cerebellar vermis.
TS20: metencephalon	Absent			No expression is seen in the prepontine hindbrain (rhombomeres 1 and 2). This includes: A4, A6 and A7 noradrenergic cell groups and median raphe nucleus (B5).
TS20: pons	Absent			No expression is seen in the pontine hindbrain (rhombomeres 3 and 4). This includes: A5 noradrenergic cell group, r4 part.
TS20: medulla oblongata	Present	Regionally restricted		Expression is seen in the pontomedullary hindbrain (rhombomeres 5 and 6) and the medullary hindbrain (rhombomeres 7-11) gigantocellular reticular area mantle zone. No expression is seen in A5 noradrenergic cell group, r5 part; raphe magnus nucleus (B3); or B1 and B2 serotonergic cell groups.
TS20: spinal cord	Present	Regionally restricted		Expression is seen in dl6.
TS20: olfactory epithelium	Present			Expression is seen in main olfactory epithelium.
TS20: nasal epithelium	Present			Expression is seen in respiratory epithelium.
TS20: vomeronasal organ	Present

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle choroid plexus epithelium	Strong		4L

Structure	Level	Pattern	Image	Note
TS28: 3rd ventricle choroid plexus	Absent
TS28: 4th ventricle choroid plexus	Absent

Structure	Cell Type	Level	Pattern	Image	Note
TS28: olfactory epithelium		Strong		6a' , 6A	There is a strong signal in the basal layer, where olfactory neural stem cells are maintained.
TS28: olfactory epithelium	olfactory epithelial supporting cell	Present		6A , 6a'	Expression is seen in the sustentacular cells of the apical layer.
TS28: vomeronasal organ sensory epithelium		Present	Regionally restricted	6A , 6a''	Expression is seen in the apical layer of the sensory epithelium, the marginal zone, and in a few cells of the basal layer.

Structure	Level	Pattern	Image	Note
TS22: nasal epithelium	Present		7B
TS22: olfactory epithelium	Present		7B
TS22: vomeronasal organ	Present		7B	Expression fills the entire apical layer and the marginal zone.

Structure	Level	Pattern	Image	Note
TS22: nasal epithelium	Present		7B'	Expression in the mutants of both sexes is comparable to that seen in wild type.
TS22: olfactory epithelium	Present		7B'	Expression in the mutants of both sexes is comparable to that seen in wild type.
TS22: vomeronasal organ	Present		7B'	Expression fills the entire apical layer and the marginal zone. Expression in the mutants of both sexes is comparable to that seen in wild type.

Structure	Level	Pattern	Image	Note
TS20: brain	Present		ISH_Dmrt3_E12_Female

Structure	Level	Pattern	Image	Note
TS20: brain	Present		ISH_Dmrt3_E12_Male

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present
TS28: hindbrain	Present