Sox9 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:229484 Conte D, et al., The apical ectodermal ridge of the mouse model of ectrodactyly Dlx5;Dlx6-/- shows altered stratification and cell polarity, which are restored by exogenous Wnt5a ligand. Hum Mol Genet. 2016 Feb 15;25(4):740-54
Assay type:	RNA in situ
MGI Accession ID:	MGI:8174537
Gene symbol:	Sox9
Gene name:	SRY (sex determining region Y)-box 9

Probe:	Sox9 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1G wild-type ©

Specimen 1G wild-type: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit 1	Present		1G wild-type
TS20: hindlimb digit 2	Present		1G wild-type
TS20: hindlimb digit 3	Present		1G wild-type
TS20: hindlimb digit 4	Present		1G wild-type
TS20: hindlimb digit 5	Present		1G wild-type
TS20: hand pre-cartilage condensation	Present	Regionally restricted	1G wild-type	Expression is in the carpal-metacarpal skeletal elements.

1G DKO ©

Specimen 1G DKO: embryonic day 12.5; Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi (more

)

Structure	Level	Pattern	Image	Note
TS20: hand pre-cartilage condensation	Present		1G DKO	In this mutant expression appeared disoriented, particularly in their most distal domain.
TS20: hindlimb digit	Present		1G DKO	In this mutant expression appeared disoriented, particularly in their most distal domain.

S3C ©

Specimen S3C: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: footplate mesenchyme	Present		S3C

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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