Antibody:

Anti-Shh (AF464)

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong	Regionally restricted	1G	Expression was strongly detected in anterior hypothalamus.
TS15: branchial arch endoderm	Strong	Regionally restricted	1G	Expression was strongly detected in pharyngeal endoderm.
TS15: Rathke's pouch	Weak	Regionally restricted	1G	Weak signal was observed in the anterior part of Rathke's pouch epithelium.
TS15: oral region ectoderm	Weak		1G

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Strong	Regionally restricted	1H	Expression was strongly detected in anterior hypothalamus.
TS17: branchial arch endoderm	Strong	Regionally restricted	1H	Expression was strongly detected in pharyngeal endoderm.
TS17: Rathke's pouch	Weak	Regionally restricted	1H	Weak signal was observed in the anterior part of Rathke's pouch epithelium.
TS17: oral region ectoderm	Weak		1H

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present		1I

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present		1J

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present		1K

Structure	Level	Pattern	Image	Note
TS26: adenohypophysis	Present	Single cells	1L

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	4C	Expression is detected in the anterior and posterior hypothalamus in the control embryos.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong	Regionally restricted	4D	Expression was lost in the anterior hypothalamus, but strong signal was detected in posterior hypothalamus of the mutant embryos.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	4G	Expression is detected in the anterior and posterior hypothalamus in the control embryos.
TS17: Rathke's pouch	Absent		4G
TS17: branchial arch endoderm	Present		4G

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	4H	Expression was lost in the anterior hypothalamus, but signal was detected in posterior hypothalamus of the mutant embryos.
TS17: Rathke's pouch	Present	Regionally restricted	4H	There is ectopic staining in the posterior region of the developing Rathke's pouch adjacent to the pharyngeal endoderm in the mutant.