Probe:	Opcml 1b probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Widespread	1G	Highly expressed throughout developing central nervous system, including telencephalic vesicles, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord.
TS20: trigeminal ganglion	Present		1G	Expression within trigeminal (V) nerve pathway.
TS20: mandibular process	Present		1G	Expression in maxillary and mandibular regions.
TS20: maxillary process	Present		1G	Expression in maxillary and mandibular regions.
TS20: nose	Weak	Regionally restricted	1G	Expression in nostrils
TS20: hindlimb	Present	Regionally restricted	1G	Expression in proximal part of hindlimb

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present		1G'	Expression within trigeminal (V) nerve pathway.
TS20: nose	Weak	Regionally restricted	1G'	Expression in nostrils

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	1G''	Expression in proximal part of hindlimb

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1N	Robust bilateral expression throughout lateral sides of spinal cord.
TS20: dorsal root ganglion	Present	Widespread	1N	Expression thoughout dorsal root ganglia.
TS20: thoracic dorsal root ganglion	Strong		1N	Comparatively higher degree of expression in thoracic dorsal root ganglion regions.
TS20: brachial plexus	Present		1N	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.
TS20: lumbosacral plexus	Present		1N	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Widespread	2G	Highly expressed throughout developing central nervous system, including telencephalic vesicles, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord.
TS21: ear	Strong	Regionally restricted	2G	Strong expression in posterior of ear.
TS21: naris	Present		2G	Expression in nostrils.
TS21: limb interdigital region mesenchyme	Present		2G

Structure	Level	Pattern	Image	Note
TS21: lens	Weak	Regionally restricted	2G'	Weak expression in peripheral area of lens.
TS21: eye muscle	Present	Regionally restricted	2G'	Expression in orbicularis oculi muscles.
TS21: eye	Present	Diffuse	2G'	Diffuse expression around eye.
TS21: ear	Strong	Regionally restricted	2G'	Strong expression in posterior of ear.
TS21: vibrissa follicle	Present	Regionally restricted	2G'	Profound expression around developing whisker follicles.

Structure	Level	Pattern	Image	Note
TS21: limb interdigital region mesenchyme	Present		2G''
TS21: mammary bud	Present	Regionally restricted	2G''	Expression in region around mammary bud.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	2N	Robust bilateral expression along length of spinal cord.
TS21: brachial plexus	Present		2N	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.
TS21: lumbosacral plexus	Present		2N	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.

Structure	Level	Pattern	Image	Note
TS21: olfactory lobe	Present
TS21: dorsal root ganglion	Present