Probe:	Ntm 1b probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Widespread	1E	Highly expressed throughout developing central nervous system, including telencephalic vesicles, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord.
TS20: trigeminal ganglion	Present		1E	Expression within trigeminal (V) nerve pathway.
TS20: facial ganglion	Present		1E	Expression in facio-acoustic ganglion (VII/VIII) complex.
TS20: vestibulo-cochlear ganglion	Present		1E	Expression in facio-acoustic ganglion (VII/VIII) complex.
TS20: inferior vagus ganglion	Weak		1E	Expression in nodose (X) ganglion.
TS20: inner ear vestibular component	Present	Regionally restricted	1E	Expression particularly strong in dorsally located vestibular region. Expression in semicircular duct.
TS20: forelimb digit	Present	Regionally restricted	1E	Expression in specifically localized to tips of digits in forelimb.
TS20: hindlimb	Present	Regionally restricted	1E	Expression in posterior part of hindlimb.

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present		1E'	Expression within trigeminal (V) nerve pathway.
TS20: facial ganglion	Present		1E'	Expression in facio-acoustic ganglion (VII/VIII) complex.
TS20: vestibulo-cochlear ganglion	Present		1E'	Expression in facio-acoustic ganglion (VII/VIII) complex.
TS20: inferior vagus ganglion	Weak		1E'	ssion in nodose (X) ganglion.
TS20: inner ear vestibular component	Present	Regionally restricted	1E'	Expression particularly strong in dorsally located vestibular region. Expression in semicircular duct.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit	Present	Regionally restricted	1E''	Expression in specifically localized to tips of digits in forelimb.
TS20: hindlimb	Present	Regionally restricted	1E''	Expression in posterior part of hindlimb.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1L	Robust bilateral expression throughout lateral sides of spinal cord.
TS20: dorsal root ganglion	Present	Widespread	1L	Expression thoughout dorsal root ganglia.
TS20: lumbar dorsal root ganglion	Strong		1L	Strong expression in thoracic and lumbar dorsal root ganglia.
TS20: thoracic dorsal root ganglion	Strong		1L	Strong expression in thoracic and lumbar dorsal root ganglia.
TS20: brachial plexus	Present		1L	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.
TS20: lumbosacral plexus	Present		1L	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Widespread	2E	Highly expressed throughout developing central nervous system, including telencephalic vesicles, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord.
TS21: naris	Present		2E	Expression in nostrils.
TS21: limb interdigital region mesenchyme	Weak		2E

Structure	Level	Pattern	Image	Note
TS21: lens	Weak	Regionally restricted	2E'	Weak expression in peripheral area of lens.
TS21: eye muscle	Present	Regionally restricted	2E'	Expression in orbicularis oculi muscles.
TS21: eye	Present	Diffuse	2E'	Diffuse expression around eye.
TS21: vibrissa follicle	Present	Regionally restricted	2E'	Profound expression around developing whisker follicles.

Structure	Level	Pattern	Image	Note
TS21: limb interdigital region mesenchyme	Present		2E''
TS21: mammary bud	Present	Regionally restricted	2E''	Expression in region around mammary bud.

Structure	Level	Pattern	Image	Note
TS21: spinal cord roof plate	Present		2L	Expression specifically marked roof plate region of spinal cord.
TS21: dorsal root ganglion	Present	Widespread	2L	Expression thoughout dorsal root ganglia.
TS21: brachial plexus	Weak		2L	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.
TS21: lumbosacral plexus	Weak		2L	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.

Structure	Level	Pattern	Image	Note
TS21: olfactory lobe	Present
TS21: spinal cord	Present	Regionally restricted		Expression throughout lateral sides of spinal cord.
TS21: ear	Weak