Probe:	Negr1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Widespread	1C	Highly expressed throughout developing central nervous system, including telencephalic vesicles, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord.
TS20: trigeminal ganglion	Weak		1C	Expression within trigeminal (V) nerve pathway.
TS20: facial ganglion	Weak		1C	Expression in facio-acoustic ganglion (VII/VIII) complex.
TS20: vestibulo-cochlear ganglion	Weak		1C	Expression in facio-acoustic ganglion (VII/VIII) complex.
TS20: inferior vagus ganglion	Weak		1C	Expression in nodose (X) ganglion.
TS20: inner ear vestibular component	Present	Regionally restricted	1C	Expression particularly strong in dorsally located vestibular region. Expression in semicircular duct.
TS20: mandibular process	Present	Regionally restricted	1C	Expression in maxillary and mandibular regions.
TS20: maxillary process	Present	Regionally restricted	1C	Expression in maxillary and mandibular regions.
TS20: vibrissa follicle	Present		1C	Expression in whisker follicles..
TS20: nose	Weak	Regionally restricted	1C	Expression in nostrils
TS20: forelimb interdigital region mesenchyme	Present	Regionally restricted	1C
TS20: hindlimb	Present	Regionally restricted	1C	Expression in anterior region and small structural subunits of hindlimb.

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Weak		1C'	Expression within trigeminal (V) nerve pathway.
TS20: facial ganglion	Weak		1C'	Expression in facio-acoustic ganglion (VII/VIII) complex.
TS20: vestibulo-cochlear ganglion	Weak		1C'	Expression in facio-acoustic ganglion (VII/VIII) complex.
TS20: inferior vagus ganglion	Weak		1C'	Expression in nodose (X) ganglion.
TS20: skeletal muscle	Present	Regionally restricted	1C'	Expression in orbicularis occuli muscle.
TS20: inner ear vestibular component	Present	Regionally restricted	1C'	Expression particularly strong in dorsally located vestibular region. Expression in semicircular duct.
TS20: vibrissa follicle	Present		1C'	Expression in whisker follicles..
TS20: nose	Weak	Regionally restricted	1C'	Expression in nostrils

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region mesenchyme	Present		1C''
TS20: hindlimb	Present	Regionally restricted	1C''	Expression in anterior region and small structural subunits of hindlimb.
TS20: mammary bud	Present		1C''	Specifically expressed within mammary bud.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1J	Robust bilateral expression throughout lateral sides of spinal cord.
TS20: spinal cord roof plate	Present		1J	Expression specifically marked roof plate region of spinal cord.
TS20: dorsal root ganglion	Present	Widespread	1J	Expression thoughout dorsal root ganglia.
TS20: brachial plexus	Present		1J	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.
TS20: lumbosacral plexus	Present		1J	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present			Expression in developing rib.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Widespread	2C	Highly expressed throughout developing central nervous system, including telencephalic vesicles, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord.
TS21: naris	Present		2C	Expression in nostrils.
TS21: limb interdigital region mesenchyme	Present		2C

Structure	Level	Pattern	Image	Note
TS21: lens	Weak	Regionally restricted	2C'	Weak expression in peripheral area of lens.
TS21: eye muscle	Present	Regionally restricted	2C'	Expression in orbicularis oculi muscles.
TS21: vibrissa follicle	Present	Regionally restricted	2C'	Expression in whisker follicles distally.

Structure	Level	Pattern	Image	Note
TS21: limb interdigital region mesenchyme	Present		2C''
TS21: mammary bud	Present	Regionally restricted	2C''	Specifically expressed within mammary bud.

Structure	Level	Pattern	Image	Note
TS21: spinal cord roof plate	Present		2J	Expression specifically marked roof plate region of spinal cord.
TS21: dorsal root ganglion	Present	Widespread	2J	Expression thoughout dorsal root ganglia.
TS21: brachial plexus	Present		2J	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.
TS21: lumbosacral plexus	Present		2J	Expression in presumptive brachial and lumbosacral plexuses or in adjacent developing skeleton.

Structure	Level	Pattern	Image	Note
TS21: olfactory lobe	Present
TS21: spinal cord	Present	Regionally restricted		Expression throughout lateral sides of spinal cord.
TS21: ear	Weak
TS21: tail	Present			Expression in tailbud region

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region mesenchyme	Present		3A	High expression in forelimb and hindlimb interdigital mesenchyme, whisker follicles, dorsal root ganglia, spinal cord, and in caudal extremity of neural tube and tail bud.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present		3B	High expression in forelimb and hindlimb interdigital mesenchyme, whisker follicles, dorsal root ganglia, spinal cord, and in caudal extremity of neural tube and tail bud.
TS21: dorsal root ganglion	Present		3B	High expression in forelimb and hindlimb interdigital mesenchyme, whisker follicles, dorsal root ganglia, spinal cord, and in caudal extremity of neural tube and tail bud.
TS21: forelimb interdigital region mesenchyme	Present		3B	High expression in forelimb and hindlimb interdigital mesenchyme, whisker follicles, dorsal root ganglia, spinal cord, and in caudal extremity of neural tube and tail bud.
TS21: hindlimb interdigital region mesenchyme	Present		3B	High expression in forelimb and hindlimb interdigital mesenchyme, whisker follicles, dorsal root ganglia, spinal cord, and in caudal extremity of neural tube and tail bud.
TS21: tail	Present		3B	High expression in forelimb and hindlimb interdigital mesenchyme, whisker follicles, dorsal root ganglia, spinal cord, and in caudal extremity of neural tube and tail bud.

Structure	Level	Pattern	Image	Note
TS21: tail	Present		3C	High expression in forelimb and hindlimb interdigital mesenchyme, whisker follicles, dorsal root ganglia, spinal cord, and in caudal extremity of neural tube and tail bud.
TS21: vibrissa follicle	Present		3C	High expression in forelimb and hindlimb interdigital mesenchyme, whisker follicles, dorsal root ganglia, spinal cord, and in caudal extremity of neural tube and tail bud.

Structure	Level	Pattern	Image	Note
TS20: vibrissa follicle	Present	Regionally restricted	5C	Expression near whisker follicular areas.
TS20: limb	Present		5C	Expression at developing limbs.
TS20: inner ear vestibular component	Present	Regionally restricted	5C	Expression in semicircular duct area.