Probe:	Lsamp universal probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Widespread	1B	Highly expressed throughout developing central nervous system, including telencephalic vesicles, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord.
TS20: trigeminal ganglion	Present		1B	Expression within trigeminal (V) nerve pathway.
TS20: inner ear vestibular component	Present	Regionally restricted	1B	Expression particularly strong in dorsally located vestibular region. Expression in semicircular duct.
TS20: face	Present	Regionally restricted	1B	Expression in a restricted area of ventral frontonasal region.
TS20: vibrissa follicle	Present	Regionally restricted	1B	Expression around developing whisker follicles.
TS20: forelimb	Present	Regionally restricted	1B	Expression in various regions of forelimb.
TS20: hindlimb	Present	Regionally restricted	1B	Primarily expressed in central part of hindlimb.

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present		1B'	Expression within trigeminal (V) nerve pathway.
TS20: lens primordium	Present		1B'
TS20: inner ear vestibular component	Present	Regionally restricted	1B'	Expression particularly strong in dorsally located vestibular region.
TS20: vibrissa follicle	Present	Regionally restricted	1B'	Expression around developing whisker follicles.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	1B''	Expression in various regions of forelimb.
TS20: hindlimb	Present	Regionally restricted	1B''	Primarily expressed in central part of hindlimb.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1I	Robust bilateral expression throughout lateral sides of spinal cord.
TS20: spinal cord roof plate	Present		1I	Expression specifically marked roof plate region of spinal cord.
TS20: dorsal root ganglion	Present	Widespread	1I	Expression thoughout dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Widespread	2B	Highly expressed throughout developing central nervous system, including telencephalic vesicles, diencephalon, mesencephalon, metencephalon, myelencephalon and spinal cord.
TS21: ear	Strong	Regionally restricted	2B	Strong expression in posterior of ear.
TS21: limb interdigital region mesenchyme	Present		2B

Structure	Level	Pattern	Image	Note
TS21: lens	Weak	Regionally restricted	2B'	Weak expression within center of lens.
TS21: eye	Present	Diffuse	2B'	Diffuse expression around eye.
TS21: ear	Strong	Regionally restricted	2B'	Strong expression in posterior of ear.
TS21: vibrissa follicle	Present	Regionally restricted	2B'	Profound expression around developing whisker follicles.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region mesenchyme	Present		2B''
TS21: hindlimb interdigital region mesenchyme	Present		2B''
TS21: mammary bud	Present	Regionally restricted	2B''	Expression in region around mammary bud.

Structure	Level	Pattern	Image	Note
TS21: spinal cord roof plate	Present		2I	Expression specifically marked roof plate region of spinal cord.
TS21: dorsal root ganglion	Present	Widespread	2I	Expression thoughout dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS21: olfactory lobe	Weak
TS21: spinal cord	Present	Regionally restricted		Expression throughout lateral sides of spinal cord.
TS21: mammary bud	Present	Regionally restricted		Expression around mammary glands.

Structure	Level	Pattern	Image	Note
TS20: vibrissa follicle	Present		5D	Expression near whisker follicular areas.
TS20: limb	Present		5D
TS20: inner ear vestibular component	Present	Regionally restricted	5D	Expression in semicircular duct area.