Antibody:	anti-4F
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Widespread	3A	Expression in most brain regions from E14. No expression in some proliferative layers.
TS22: brainstem	Strong		3A	Expression especially prominent in olfactory bulb, cerebral cortex, hippocampus and brainstem.
TS22: cerebral cortex	Strong		3A	Expression especially prominent in olfactory bulb, cerebral cortex, hippocampus and brainstem.
TS22: hippocampus	Strong		3A	Expression especially prominent in olfactory bulb, cerebral cortex, hippocampus and brainstem.
TS22: main olfactory bulb	Strong		3A	Expression especially prominent in olfactory bulb, cerebral cortex, hippocampus and brainstem.
TS22: cortical plate	Strong		3A
TS22: nerve fiber tract	Present	Regionally restricted	3A	Expression in regions rich in oligodendroglial precursors, such a mamillothalamic tract.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present		3B
TS26: hippocampus	Strong	Regionally restricted	3B	Strong expression in hippocampus. Expression in embryonic fimbria.
TS26: hypothalamus	Trace	Widespread	3B	Faint widespread expression at embryonic stages.

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present	Regionally restricted	3C
TS28: hippocampus	Strong		3C	Significant expression in adult.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Strong		3D	High expression between E16 and P0.
TS24: cortical plate	Present		3D

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	3E	Strong expression in hippocampus. Expression in embryonic fimbria.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3F	Significant expression at P15.

Structure	Level	Pattern	Image	Note
TS27: corpus callosum	Present	Regionally restricted	3G	Expression in cells of oligodendroglial shape as early as P0 in corpus callosum.
TS27: telencephalon septum	Present		3G

Structure	Level	Pattern	Image	Note
TS27: telencephalon subventricular zone	Strong	Regionally restricted	3H	Strong expression in anterior subventricular zone around P0.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: telencephalon subventricular zone		Present	Regionally restricted	3I	Expression in anterior subventricular zone in adult.
TS28: corpus callosum	oligodendrocyte	Present	Regionally restricted	3I	Expression in mature oligodendrocytes in corpus callosum.

Structure	Level	Pattern	Image	Note
TS28: entorhinal cortex	Strong		3J	Expression especially outstanding in entorhinal cortex.

Structure	Level	Pattern	Image	Note
TS27: corpora quadrigemina	Strong		3K	Prominent expression in oculomotor nucleus, periaqueductal gray area, colliculi, raphe and pararubral nuclei.
TS27: oculomotor nucleus	Strong		3K	Prominent expression in oculomotor nucleus, periaqueductal gray area, colliculi, raphe and pararubral nuclei.
TS27: periaqueductal grey	Strong		3K	Prominent expression in oculomotor nucleus, periaqueductal gray area, colliculi, raphe and pararubral nuclei.
TS27: raphe nucleus	Strong		3K	Prominent expression in oculomotor nucleus, periaqueductal gray area, colliculi, raphe and pararubral nuclei.

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Strong		3L	Expression especially prominent in olfactory bulb.
TS22: facial nucleus	Present		3L

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present		3M

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		3N	Expression in olfactory bulb, mainly in mitral cell layer and glomerular layer.
TS28: main olfactory bulb mitral cell layer	Present		3N	Expression in olfactory bulb, mainly in mitral cell layer and glomerular layer.

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present	Regionally restricted	3O	Expression in early postnatal hypothalamus, higher in particular areas at postnatal stages. Remarkable intensity of expression near arcuate and posterior hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3P

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Widespread	3Q	Expression in most cell types of cerebellum.v

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present		5A	Expression in early postnatal hypothalamus

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Present		5C , 5B	Expression in cells near arcuate nucleus.

Structure	Level	Pattern	Image	Note
TS27: corpus callosum	Present		5F

Structure	Level	Pattern	Image	Note
TS27: anterior commissure	Present		5G	Expression in cells of oligodendroglial shape as early as P0.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: corpus callosum	oligodendrocyte	Present		5I	Expression in mature oligodendrocytes.