Gene Expression Data
Assay Details
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Assay
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Reference:
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J:55594
Iseki S, et al., Fgfr1 and Fgfr2 have distinct differentiation- and proliferation-related roles in the developing mouse skull vault. Development. 1999 Dec;126(24):5611-20
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7856901
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Gene symbol:
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Sparc
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Gene name:
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secreted acidic cysteine rich glycoprotein
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Probe:
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osteonectin cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2K:
embryonic day 16.0
(more  )
Specimen
2K:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: frontal bone |
preosteoblast |
Present |
Regionally restricted |
2K
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Expression is in cells on the outer (skin side) surface of the osteoid, and in preosteoblasts at the sutural margin of the osteoid plate.
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| TS24: parietal bone |
preosteoblast |
Present |
Regionally restricted |
2K
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Expression is in cells on the outer (skin side) surface of the osteoid, and in preosteoblasts at the sutural margin of the osteoid plate.
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Specimen
4C:
embryonic day 16.0
(more )
Specimen
4C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: frontal bone |
Present |
Regionally restricted |
4C
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Expression is strongest in the cells of the outer (skin side) layer, and is low or undetectable in the internal cells.
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Analysis Tools
