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Gene Expression Data
Assay Details
Assay
Reference: J:208419 Basta JM, et al., Sall1 balances self-renewal and differentiation of renal progenitor cells. Development. 2014 Mar;141(5):1047-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7855594
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: anti-WT-1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3B +/+: embryonic day 13.5 (more )
Note: Double labeled: green - Wt1; red - Cytokeratin.
Structure Level Pattern Image Note
TS21: renal vesicle Present Regionally restricted 3B WT1/Cyto +/+ Expression formed a c-shaped pattern of presumptive podocytes.
Specimen 3B -/-: embryonic day 13.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Wt1; red - Cytokeratin.
Structure Level Pattern Image Note
TS21: renal vesicle Present Regionally restricted 3B WT1/Cyto -/- In the mutant, renal vesicles were immunopositive, but the normal C-shaped pattern of presumptive podocytes was not apparent.
Specimen S1 +/+: embryonic day 16.5 (more )
Note: Double labeled: green - Wt1; red - Cytokeratin.
Structure Level Pattern Image Note
TS24: maturing glomerular tuft Present S1_WT1/Cyto +/+
Specimen S1 -/-: embryonic day 16.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Wt1; red - Cytokeratin.
Structure Level Pattern Image Note
TS24: nephrogenic zone Present Regionally restricted S1_WT1/Cyto -/- Immunopositive structures were observed toward the periphery of the kidney in the nephrogenic zone.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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