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Gene Expression Data
Assay Details
Assay
Reference: J:208419 Basta JM, et al., Sall1 balances self-renewal and differentiation of renal progenitor cells. Development. 2014 Mar;141(5):1047-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7855585
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Antibody: Anti-NCAM
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 3A E11.5 +/-: embryonic day 11.5; Sall1tm3Ryn/Sall1+ (more )
Note: Double labeled: red - Ncam1 and Ncam2; green - Lef1 (assay MGI:7855584).
Structure Level Pattern Image Note
TS19: metanephros Present Regionally restricted 3A Lef1/NCAM - E11.5 +/- Lef1-positive;NCAM1/2-positive structures were observed with a morphologically distinct lumen.
Specimen 3A E12.5 -/-: embryonic day 12.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: red - Ncam1 and Ncam2; green - Lef1 (assay MGI:7855584).
Structure Level Pattern Image Note
TS20: metanephros Present Regionally restricted 3A Lef1/NCAM - E12.5 -/- There were significantly more Lef1-positive;NCAM1/2-positive structures with a morphologically distinct lumen in the mutant compared with control.
Specimen 3B +/+: embryonic day 13.5 (more )
Note: Double labeled: red - Ncam1 and Ncam2; green - Pax2 (assay MGI:7855588).
Structure Level Pattern Image Note
TS21: renal vesicle Present Regionally restricted 3B Pax2/NCAM +/+ Immunopositive renal vesicles formed on the ventral side of the ureter, towards the interior of the kidney.
Specimen 3B -/-: embryonic day 13.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: red - Ncam1 and Ncam2; green - Pax2 (assay MGI:7855588).
Structure Level Pattern Image Note
TS21: renal vesicle Present Regionally restricted 3B Pax2/NCAM -/- In the mutant, some renal vesicles were located ectopically on the dorsal side of the ureter, towards the periphery of the forming kidney; these renal vesicle structures were immunopositive.
Specimen S1 +/+ CDH6/NCAM: embryonic day 16.5 (more )
Note: Double labeled: red - Ncam1 and Ncam2; green - Cdh6 (assay MGI:7855606).
Structure Level Pattern Image Note
TS24: metanephros Present S1_Cdh6/NCAM +/+
Specimen S1 -/- CDH6/NCAM: embryonic day 16.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: red - Ncam1 and Ncam2; green - Cdh6 (assay MGI:7855606).
Structure Level Pattern Image Note
TS24: metanephros Present S1_Cdh6/NCAM -/-
Specimen S1 +/+ LTL/NCAM: embryonic day 16.5 (more )
Note: Double labeled: red - Ncam1 and Ncam2; green - LTL.
Structure Level Pattern Image Note
TS24: metanephros Present S1_LTL/NCAM +/+
Specimen S1 -/- LTL/NCAM: embryonic day 16.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: red - Ncam1 and Ncam2; green - LTL.
Structure Level Pattern Image Note
TS24: metanephros Present S1_LTL/NCAM -/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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