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Gene Expression Data
Assay Details
Assay
Reference: J:350265 Feng H, et al., A new Prdm1-Cre line is suitable for studying the second heart field development. Dev Biol. 2024 Jun 14;514:78-86
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7852520
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: anti-Islet1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5K,K',K'': embryonic day 9.5 (more )
Note: Double labeled: red - Isl1; white - Mki67 (assay MGI:7852521).
Structure Level Pattern Image Note
TS15: secondary heart field Present Patchy 5K , 5K' Expression in secondary heart field progenitors in dorsal pericardial wall.
TS15: outflow tract Absent 5K , 5K''
Specimen 5L,L',L'': embryonic day 9.5; Prdm1em1(cre)Zzy/Prdm1+, Tbx20em1Gpt/Tbx20em1Gpt (more )
Note: Conditional mutant. Double labeled: red - Isl1; white - Mki67 (assay MGI:7852521).
Structure Level Pattern Image Note
TS15: secondary heart field Weak Regionally restricted 5L' , 5L Significantly reduced expression in secondary heart field progenitors in dorsal pericardial wall.
TS15: outflow tract Present 5L'' , 5L Increased Isl1+Mki67+ double expressing cells compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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