Gene Expression Data
Assay Details
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Assay
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Reference:
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J:171050
Thiagarajan RD, et al., Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways. PLoS One. 2011;6(2):e17286
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7840437
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Gene symbol:
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Vip
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Gene name:
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vasoactive intestinal polypeptide
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Probe:
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RIKEN clone 9130007F05 subclone
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Authors described this gene as an 'anchor gene', which was defined as "genes whose expression is absolutely specific to a given anatomical compartment and/or developmental stage" in mouse kidney development.
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Results
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Specimen
Supplementary Table 1:
embryonic day 15.5
(more  )
Specimen
Supplementary Table 1:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: renal corpuscle |
Present |
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Section in situ hybridization confirmed specific expression in renal corpuscle. Validated anchor gene. Expression was restricted to the more mature Stage IV renal corpuscle. Expression marked the juxtaglomerular arterioles.
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Analysis Tools
