Probe:	Nrp1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	4B , 4F	Expression in two distinct groups of cells corresponding to trigeminal and hyoid cranial neural crest cells
TS15: otocyst	Present		4B , 4F
TS15: midbrain-hindbrain junction	Weak		4B , 4F	Weak expression at midbrain-hindbrain boundary

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	4C , 4G	Expression in two distinct groups of cells corresponding to trigeminal and hyoid cranial neural crest cells.
TS15: otocyst	Present		4C , 4G
TS15: midbrain-hindbrain junction	Weak		4G , 4C	Weak expression at midbrain-hindbrain boundary

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Weak	Regionally restricted	4H , 4L	Marked reduction of expression within subpopulation of Sox10-expressing trigeminal cranial neural crest cells. Expression in hyoid cranial neural crest cell stream appears unaffected compared to wild type.
TS15: otocyst	Present		4L , 4H	Expression in otic vesicle unaffected compared to wild type.
TS15: midbrain-hindbrain junction	Present	Regionally restricted	4L , 4H	Expression at midbrain-hindbrain boundary expanded caudally through rhombomere 1.
TS15: rhombomere 1	Present		4L , 4H	Ectopic expression throughout rhombomere 1.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Weak	Regionally restricted	4I , 4M	Marked reduction of expression within subpopulation of Sox10-expressing trigeminal cranial neural crest cells. Expression in hyoid cranial neural crest cell stream appears unaffected compared to wild type.
TS15: otocyst	Present		4I , 4M	Expression in otic vesicle unaffected compared to wild type.
TS15: midbrain-hindbrain junction	Weak	Regionally restricted	4I , 4M	Expression at midbrain-hindbrain boundary expanded caudally through rhombomere 1.
TS15: rhombomere 1	Present		4I , 4M	Ectopic expression throughout rhombomere 1.