Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:51034
Kliewer SA, et al., An orphan nuclear receptor activated by pregnanes defines a novel steroid signaling pathway. Cell. 1998 Jan 9;92(1):73-82
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:7798875
|
Gene symbol:
|
Nr1i2
|
Gene name:
|
nuclear receptor subfamily 1, group I, member 2
|
Probe:
|
PXR probe 1
|
Probe preparation:
|
labelled with S35
|
Visualized with:
|
Autoradiography
|
Assay notes:
|
Three Nr1i2 probes were described, but the one used in this assay was not detailed. PXR probe 2 MGI:7798870 (recognizing only PXR.1) or PXR probe 3 MGI:7798871 (recognizing both PXR.1 and PXR.2) may have been used. Similar results were obtained with all three probes. However, signal intensity was consistently higher with probes that recognize both transcripts.
|
|
Results
|
Specimen
2C:
embryonic day 18.0
(more )
Specimen
2C:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA * NMRI
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: liver |
Present |
|
2C
|
|
Specimen
2D:
embryonic day 18.0
(more )
Specimen
2D:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA * NMRI
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 18.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA * NMRI
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
|