Gene Expression Data
Assay Details
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Assay
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Reference:
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J:352610
Ikeda S, et al., Harderian Gland Development and Degeneration in the Fgf10-Deficient Heterozygous Mouse. J Dev Biol. 2024 Jun 3;12(2)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7798841
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Gene symbol:
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Fgfr2
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Gene name:
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fibroblast growth factor receptor 2
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Probe:
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Fgfr2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3C/S8C:
embryonic day 15.0
(more  )
Specimen
3C/S8C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3H:
embryonic day 15.0
(more )
Specimen
3H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4K/S9B/C:
embryonic day 19.0; Fgf10tm1Ska/Fgf10 +
(more )
Specimen
4K/S9B/C:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Fgf10tm1Ska/Fgf10+
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: eye epithelium |
Present |
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4 Cont._K
,
S9C
,
S9B
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Expressed in the invaginating epithelium flanking the nascent nictitating membrane. Expression indicates that Harderian gland cells did not reach the Harderian gland mesenchyme. Different expression levels were observed depending on the epithelia.
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Specimen
S9D:
embryonic day 19.0; Fgf10tm1Ska/Fgf10 +
(more )
Specimen
S9D:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Fgf10tm1Ska/Fgf10+
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: eye epithelium |
Present |
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S9D
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A small population of cells without expression was found in the Harderian gland mesenchyme in a more rostral section.
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Specimen
S9E:
embryonic day 19.0; Fgf10tm1Ska/Fgf10 +
(more )
Specimen
S9E:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Fgf10tm1Ska/Fgf10+
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: perioptic mesenchyme |
Present |
Scattered |
S9E
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A few positive cells were scattered in the Harderian gland mesenchyme in a more caudal section.
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Specimen
S11D:
embryonic day 19.0
(more )
Specimen
S11D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S12C:
embryonic day 19.0; Fgf10tm1Ska/Fgf10 +
(more )
Specimen
S12C:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Fgf10tm1Ska/Fgf10+
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: eye epithelium |
Present |
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S12C
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No positive Harderian gland cells were seen in this heterozygous mutant.
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Specimen
S12F:
embryonic day 19.0; Fgf10tm1Ska/Fgf10 +
(more )
Specimen
S12F:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Fgf10tm1Ska/Fgf10+
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: eye epithelium |
Present |
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S12F
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No positive Harderian gland cells were seen in this heterozygous mutant.
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Analysis Tools