T RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:254597 Bhargava S, et al., The epigenetic modifier Fam208a is required to maintain epiblast cell fitness. Sci Rep. 2017 Aug 24;7(1):9322
Assay type:	RNA in situ
MGI Accession ID:	MGI:7798001
Gene symbol:	T
Gene name:	brachyury, T-box transcription factor T

Probe:	T probe
Probe preparation:	RNA

Results

S2A ©

Specimen S2A: embryonic day 6.5 (more

)
Note: Early streak stage. Double labeled: blue - T; blue - Elf5 (assay MGI:7797997).

Structure	Level	Pattern	Image	Note
TS8: extraembryonic ectoderm	Present		S2A

S2A' ©

Specimen S2A': embryonic day 6.5; Tasor^MommeD6/Tasor^MommeD6 (more

)
Note: Prestreak stage. Double labeled: blue - T; blue - Elf5 (assay MGI:7797997).

Structure	Level	Pattern	Image	Note
TS8: extraembryonic ectoderm	Present		S2A'	Minimal changes in expression compared to wild type.

1A ©

Specimen 1A: embryonic day 7.5 (more

)
Note: Late pre-headfold stage.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1A	Expression extends past distal tip of embryo and into notochord precursor.
TS11: notochordal process	Present		1A	Expression extends past distal tip of embryo and into notochord precursor.

1A' ©

Specimen 1A': embryonic day 7.5; Tasor^MommeD6/Tasor^MommeD6 (more

)
Note: Late streak/early bud stage.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	1A'	Expression restricted to posterior of embryo, extending distally 1/3rd length of epiblast, never reaching distal tip. No distal and anterior expression.

S3B ©

Specimen S3B: embryonic day 7.5; Tasor^MommeD20/Tasor^MommeD20 (more

)
Note: Late streak/early bud/early pre-headfold.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		S3B	Authors report arrested expression.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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