Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:254597 Bhargava S, et al., The epigenetic modifier Fam208a is required to maintain epiblast cell fitness. Sci Rep. 2017 Aug 24;7(1):9322
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7797985
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Oct3/4 (C-10; sc-5279)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

4A +/+ Oct4 ©

Specimen 4A +/+: embryonic day 6.5 (more

)
Note: Early streak stage.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		4A +/+ DAPI/Oct4 , 4A +/+ Oct4

4A D6/+ Oct4 ©

Specimen 4A D6/+: embryonic day 6.5; Tasor^MommeD6/Tasor⁺ (more

)
Note: Midstreak stage.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present		4A D6/+ Oct4 , 4A D6/+ DAPI/Oct4

Specimen 4A D6/D6: embryonic day 6.5; Tasor^MommeD6/Tasor^MommeD6 (more

)
Note: Early streak stage.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		4A D6/D6 Oct4 , 4A D6/D6 DAPI/Oct4	Authors report smaller expression domain compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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