Antibody:	Anti-E-Cadherin (610181; Clone 36)
Detection system:	Secondary antibody coupled to Alexa Fluor 555

Structure	Cell Type	Level	Pattern	Image	Note
TS14: 2nd branchial arch	epithelial cell	Present		1a

Structure	Cell Type	Level	Pattern	Image	Note
TS14: 3rd branchial arch	epithelial cell	Present		1b' , 1b	The first indication of morphogenesis of the 3rd pharyngeal arch was seen.
TS14: 2nd branchial arch	epithelial cell	Present		1b' , 1b
TS14: 3rd branchial arch endoderm	epithelial cell	Present		1b , 1b'	The endodermal epithelium began to bend.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: 3rd branchial arch endoderm	epithelial cell	Present		1c	A 2nd bending occurred at a position posterior to the 1st bending site.
TS15: branchial arch	epithelial cell	Present		1c

Structure	Cell Type	Level	Pattern	Image	Note
TS15: 3rd branchial arch endoderm	epithelial cell	Present		1d	A 2nd bending occurred at a position posterior to the 1st bending site.
TS15: branchial arch	epithelial cell	Present		1d

Structure	Cell Type	Level	Pattern	Image	Note
TS15: 3rd branchial arch endoderm	epithelial cell	Present		1e	A third bending took place at the position where the first bending had occurred.
TS15: branchial arch	epithelial cell	Present		1e

Structure	Cell Type	Level	Pattern	Image	Note
TS15: 3rd branchial arch endoderm	epithelial cell	Present		1f	A third bending took place at the position where the first bending had occurred, which connected the endoderm and the ectoderm epithelium, leading to the completion of the formation of the third pharyngeal pouch.
TS15: branchial arch	epithelial cell	Present		1f

Structure	Cell Type	Level	Pattern	Image	Note
TS14: 2nd branchial arch	epithelial cell	Present		1l	Similar as in the wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS14: 3rd branchial arch endoderm	epithelial cell	Present		1n , 1n'	Morphological abnormality of the epithelial sheet was first observed, although the 1st bending of the endoderm sheet was observed. The epithelial sheet did not maintain the monolayer structure at the bending site.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: 3rd branchial arch endoderm	epithelial cell	Present		1m	The 2nd bending of the endoderm sheet was observed; but again the epithelial morphology of the endoderm appeared to be partly disturbed.
TS15: branchial arch	epithelial cell	Present		1m

Structure	Cell Type	Level	Pattern	Image	Note
TS15: 3rd branchial arch endoderm	epithelial cell	Present		1o	The 2nd bending of the endoderm sheet was observed; but again the epithelial morphology of the endoderm appeared to be partly disturbed.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: branchial arch	epithelial cell	Present		1p	Severe defect in the morphology of the 3rd and 4th pharyngeal arches, especially in the development of the mesenchyme in these arches.