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Gene Expression Data
Assay Details
Assay
Reference: J:173838 Manuylov NL, et al., Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol. 2011 May 15;353(2):229-41
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7783744
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: anti-VASA/DDX4
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 10F XY FOG2cKO left: embryonic day 16.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Ddx4; green - Gata4 (assay MGI:7782574).
Structure Level Pattern Image Note
TS24: germ cell of gonad Present 10F left
Specimen 10F XY FOG2cKO right: embryonic day 16.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Ddx4; green - Gata4 (assay MGI:7782574).
Structure Level Pattern Image Note
TS24: germ cell of gonad Present 10F right
Specimen S1A/C control: postnatal day 45 (more )
Note: Double labeled: red - Ddx4; green - Gata4 (assay MGI:7783737).
Structure Level Pattern Image Note
TS28: germ cell of testis Present S1C left , S1A left
TS28: spermatozoon Present S1C left , S1A left
Specimen S1A/C GATA4cKO: postnatal day 45; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Gata4 (assay MGI:7783737).
Structure Level Pattern Image Note
TS28: testis Absent S1A right , S1C right The testicle germinal epithelium in the mutants was disorganized and no germ cells or mature sperm were observed.
Specimen S5H XY FOG2cKO: postnatal day 1; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Ddx4; green - Foxl2 (assay MGI:7783742).
Structure Level Pattern Image Note
TS27: germ cell of gonad Present S5H Germ cells were associated with the remaining testis cords.
Specimen S5I XX control: postnatal day 1 (more )
Note: Double labeled: red - Ddx4; green - Foxl2 (assay MGI:7783742).
Structure Level Pattern Image Note
TS27: germ cell of ovary Present S5I
Specimen S5K XY FOG2cKO: postnatal day 21; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite.
Structure Level Pattern Image Note
TS28: germ cell of gonad Present S5K Despite the overall "ovarian" appearance of the XY mutant gonad, the early stage follicles present in the normal ovary were absent.
Specimen S5L XX control: postnatal day 21 (more )
Note: Double labeled: red - Ddx4; green - Foxl2 (assay MGI:7783742).
Structure Level Pattern Image Note
TS28: germ cell of ovary Present S5L
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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