About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:173838 Manuylov NL, et al., Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol. 2011 May 15;353(2):229-41
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7783652
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: anti-SOX9
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3A control: embryonic day 12.5 (more )
Note: Double labeled: red - Sox9; green - Gata4 (assay MGI:7782574).
Structure Cell Type Level Pattern Image Note
TS20: Sertoli cell Sertoli cell Present 3A Control Coexpressed with Gata4.
Specimen 3A GATA4cKO: embryonic day 12.5; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Sox9; green - Gata4 (assay MGI:7782574).
Structure Cell Type Level Pattern Image Note
TS20: Sertoli cell Sertoli cell Present 3A cKO Expressed normally in the mutant.
Specimen 4B control: embryonic day 18.5; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Sox9; green - Pecam1 (assay MGI:7782572).
Structure Cell Type Level Pattern Image Note
TS26: Sertoli cell Sertoli cell Present 4B
Specimen 4E GATA4cKO: embryonic day 18.5; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Sox9; green - Pecam1 (assay MGI:7782572).
Structure Cell Type Level Pattern Image Note
TS26: Sertoli cell Sertoli cell Present 4E Normal expression, but testis cords in the mutant appeared highly irregular.
Specimen 9A control: embryonic day 14.5 (more )
Note: Double labeled: red - Sox9; green - Pecam1 (assay MGI:7782572).
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 9A
Specimen 9B FOG2cKO: embryonic day 14.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Sox9; green - Pecam1 (assay MGI:7782572).
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 9B A dramatic down-regulation was seen.
Specimen 9C control: postnatal day 1 (more )
Structure Cell Type Level Pattern Image Note
TS27: Sertoli cell Sertoli cell Present 9C
Specimen 9D FOG2cKO: postnatal day 1; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite.
Structure Cell Type Level Pattern Image Note
TS27: Sertoli cell Sertoli cell Present 9D A dramatic down-regulation was seen.
Specimen 10D FOG2cKO left: embryonic day 16.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Sox9; green - Foxl2 (assay MGI:7783742).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 10D left
Specimen 10D FOG2cKO right: embryonic day 16.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Sox9; green - Foxl2 (assay MGI:7783742).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 10D right SOX9 and FOXL2 expression was not segregated spatially, the proteins were not expressed in the same cell.
Specimen S5D/E/F XY FOG2cKO: postnatal day 1; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Sox9; green - Foxl2 (assay MGI:7783742).
Structure Level Pattern Image Note
TS27: gonad Present S5D , S5E , S5F
Specimen S5G XX control: postnatal day 1 (more )
Note: Double labeled: red - Sox9; green - Foxl2 (assay MGI:7783742).
Structure Level Pattern Image Note
TS27: ovary Absent S5G
Specimen S5J XY FOG2cKO: postnatal day 21; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Sox9; green - Foxl2 (assay MGI:7783742).
Structure Level Pattern Image Note
TS28: gonad Present S5J Partial sex reversal, with overall "ovarian" appearance.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory